CRM197对人乳腺癌细胞侵袭及转移的抑制作用及机制  

The inhibitory effects and mechanism of CRM197 on the migration and invasion of human breast cancer cells

作　　者：梁秋实[1] 胡宜[2] 

机构地区：[1]中国医科大学附属盛京医院超声科,辽宁沈阳110004 [2]中国医科大学附属盛京医院神经外科,辽宁沈阳110004

出　　处：《转化医学电子杂志》2017年第2期26-29,共4页E-Journal of Translational Medicine

基　　金：国家自然科学基金(81302191)

摘　　要：目的:研究CRM197对人乳腺癌细胞侵袭、转移能力以及对基质金属铁蛋白酶-2、9活性和磷酸化Akt表达的影响.方法:将浓度为10μg/m L的CRM197孵育MDA-MB-231人乳腺癌细胞24 h.利用Transwell小室建立体外迁移及侵袭模型,观察CRM197对MDA-MB-231细胞迁移及侵袭能力的影响.利用明胶酶谱分析方法检测CRM197对MDA-MB-231细胞的基质金属铁蛋白酶-2、9活性改变情况.最后利用Western blot方法分析CRM197对MDA-MB-231细胞的磷酸化Akt表达水平的影响.结果:在10μg/m L的CRM197处理下,MDA-MB-231人乳腺癌细胞的侵袭和转移能力明显降低,同时基质金属铁蛋白酶-2、9活性以及磷酸化Akt的表达水平受到显著抑制.结论:CRM197能够通过抑制基质金属铁蛋白酶-2和9的活性而减弱人乳腺癌细胞的体外侵袭及迁移特性,并且在这一过程中PI3K/Akt信号通路发挥作用.AIM： To investigate the effects of CRM197 on the migration,invasiveness,the activity of matrix metalloproteinase-2,9 as well as the expression of phosphorylated Akt of human glioma cells. METHODS： MDA-MB-231 human breast cancer cells were treated with 10 μg / m L CRM197 for 24 h. Cellular migration and invasiveness were assessed with Transwell assays. Zymography assay was performed to check the enzymatic activity of matrix metalloproteinase-2 and 9. Western blos assay was carried out to investigate the expression of phosphorylated Akt. RESULTS：Under the treatment of 10 μg / m L CRM197,the migration and invasion of MDA-MB-231 human breast cancer cells reduced significantly. The activity of matrix metalloproteinase- 2 / 9 and expression of phosphorylated Akt were inhibited significantly as well. CONCLUSION： CRM197 possibly inhibite the migration and invasiveness of human glioma cells through inhibiting the activity of matrix metalloproteinase-2 and 9,and PI3 K / Akt pathway may be involved in the process.

关 键 词：CRM197 乳腺癌 侵袭 迁移 基质金属铁蛋白酶 AKT 

分 类 号：R737.9[医药卫生—肿瘤]