木犀草苷对3T3-L1前脂肪细胞分化的抑制作用  

Inhibitory effect of galuteolin on the differentiation of 3T3-L1 preadipocyte

作　　者：李慧玲[1] 吴骁[2] 王丹 张秋华[1] 

机构地区：[1]辽宁中医药大学基础药理教研室,辽宁沈阳110847 [2]辽宁何氏医学院实验动物中心,辽宁沈阳110163 [3]锦州医科大学基础医学院组胚教研室,辽宁锦州121001

出　　处：《中国医药导报》2017年第2期16-19,共4页China Medical Herald

基　　金：辽宁省沈阳市科技计划项目(F16-205-1-31)

摘　　要：目的观察木犀草苷对3T3-L1前脂肪细胞分化的抑制作用。方法用噻唑蓝(MTT)法检测木犀草苷对3T3-L1前脂肪细胞增殖的影响;用鸡尾酒法诱导3T3-L1细胞分化,用油红O染色、三酰甘油含量检测脂肪细胞分化程度;采用RT-PCR和Western blot方法检测木犀草苷对脂肪细胞过氧化物酶体增殖物活化受体γ(PPARγ)、CCAAT/增强子结合蛋白α(C/EBPα)m RNA和蛋白表达的影响。结果木犀草苷5~80μmol/L不影响细胞活性,160~320μmol/L显著抑制细胞活性,呈剂量依赖性抑制3T3-L1前脂肪细胞的分化,显著降低脂肪细胞PPARγ、C/EBPαm RNA和蛋白的表达。结论木犀草苷能抑制3T3-L1前脂肪细胞分化,减少脂质聚集,是通过下调PPARγ、C/EBPα的表达实现的。Objective To observe the inhibitory effect of galuteolin on the differentiation of 3T3-L1 preadipocyte. Methods The effect of galuteolin on the proliferation of 3T3-L1 preadipocyte was detected by methyl thiazolyl tetrazoli- um （MTT）; the differentiation of 3T3-L1 preadipocyte was induced by the cocktail method, the differentiation degree of preadipocyte was detected by oil red O staining and the content of triacylglycerol; the effects of galuteolin for the mR- NA and protein expression of peroxisome proliferator activated receptor-γ（PPARα） and CCAAT/enhancer binding pro- tein α （C/EBPa） were measured by RT-PCR and Western blot. Results The concentration of 5-80 μmol/L galuteolin did not affect the cell activity, 160-320μmol/Lgaluteolin could significantly inhibit the cell activity, which could sig- nificantly inhibit the differentiation of 3T3-L1 preadipocyte in a dose-dependent manner and decrease the mRNA and protein expression of PPARY and C/EBPα. Conclusion Galuteolin can inhibit the differentiation of 3T3-L1 preadipocyte, reduce the lipid accumulation, which may be realized by down-regulating the expression of PPARY and C/EBPα.

关 键 词：木犀草苷 3T3-L1前脂肪细胞 分化 PPARY C/EBPa 

分 类 号：R285[医药卫生—中药学]