AQP9在APP/PS1双转基因小鼠海马中的表达变化  

Changes in expression of Aquaporin-9 in the hippocampus of APP/PS1 double transgenic mouse model of Alzheimer's disease

作　　者：陆蔚天[1] 黄娟[1] 李昱[1] 孙善全[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆400016

出　　处：《重庆医科大学学报》2017年第1期120-124,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81601051);重庆市基础与前沿研究计划资助项目(编号:cstc2016jcyj A0172);重庆市教委科学技术研究资助项目(编号:KJ1600222);重庆市渝中区科技计划资助项目(编号:20140115);重庆医科大学2015年度国家自然科学基金预研资助项目(编号:NSFYY201525)

摘　　要：目的:研究水通道蛋白9(aquaporin-9,AQP9)在阿尔茨海默病(Alzheimer’s disease,AD)模型小鼠海马中的表达变化。方法:9月龄APPswe/PS1d E9双转基因雄性小鼠和C57野生型雄性小鼠各12只,分别为AD模型组和对照组,采用水迷宫实验检测2组小鼠学习记忆能力,采用免疫组化、免疫印迹和q RT-PCR方法检测AQP9蛋白及其m RNA在以上2种小鼠海马中的表达。结果:水迷宫实验显示,与对照组比较,模型组小鼠找到平台的潜伏期显著加长,穿越平台次数明显减少(t=7.064,P=0.000)。免疫组化、免疫印迹和q RT-PCR结果分别显示:与对照组比较,AQP9的吸光度(absorbance,A)值在海马不同区域均增加(CA1:t=7.373,P=0.000;CA2:t=3.1,P=0.011;CA3:t=4.428,P=0.001;齿状回:t=5.285,P=0.000);AQP9蛋白表达增高(t=5.305,P=0.003);AQP9 m RNA表达水平增高(t=3.646,P=0.045)。结论:在APPswe/PS1d E9双转基因AD模型小鼠海马中,AQP9表达上调。Objective：To investigate the changes in expression of Aquaporin-9（AQP9） in the hippocampus of mouse model of Alzheimer＇s disease（AD）. Methods：APPswe/PS1 d E9 double transgenic male mice aged 9 months（n=12）and C57 wild-type（WT）male mice aged 9 months（n=12）were used as model of AD and control,respectively. Morris water maze tests were used to assess the learning and memory abilities of animals. Immunohistochemical staining,Western blot and q RT-PCR were applied to exam the expression of AQP9 protein and its m RNA in the hippocampus of the AD model and WT mice. Results：The water maze test showed that AD model mice took longer latency and had significantly less platform-passing times than WT mice（t=7.064,P=0.000）. The results of immunohistochemical staining showed that the absorbance（A）value of AQP9 immunoreactivity in different hippocampus areas were increased in the hippocampus of the AD model mice compared with in that of the WT mice（CA1：t=7.373,P=0.000;CA2：t=3.1,P=0.011;CA3：t=4.428,P=0.001;girus：t=5.285,P=0.000）. The results of Western blot showed that the expression of AQP9 protein were increased compared with in that of the WT mice（t=5.305,P=0.003）. The q RT-PCR showed that AQP9 m RNA were increased in the hippocampus of the AD model mice compared with in that of the WT mice（t=3.646,P=0.045）. Conclusion：AQP9 protein and its m RNA are upregulated in the hippocampus of the APPswe/PS1 d E9 double transgenic mice model of AD.

关 键 词：水通道蛋白9 阿尔茨海默病 APPswe/PS1d E9转基因小鼠 海马 

分 类 号：R741.02[医药卫生—神经病学与精神病学]