高效液相色谱法测定饮料中苯甲酸、山梨酸钾  

Determination of Benzoic Acid,Potassium Sorbate in Beverage by High Performance Liquid Chromatography Method

作　　者：敬小丽[1] 余蓉[1] 李莉[1] 程杭杭[1] 刘能[1] 江朝[1] 高丽霞[1] 

机构地区：[1]成都中医药大学医学技术学院,成都611137

出　　处：《预防医学情报杂志》2017年第3期212-215,共4页Journal of Preventive Medicine Information

基　　金：成都中医药大学教改课题资助:卫生检验仪器学课程体系构建(编号:JGYB201533);成都中医药大学教学改革研究项目:医学检验技术平台下卫生检验专业学生实践技能差异化教学体系的建立(编号:JGZD201411)

摘　　要：目的建立了饮料中苯甲酸、山梨酸钾两种防腐剂同时检测的高效液相色谱法。方法饮料经前处理后,由C18反相色谱柱进行分离,用流动相:甲醇和0.02mol/ml的乙酸铵溶液以5∶95为比例洗脱,在流速1.0ml/min,波长230nm下进行检测,进样量为10μl,以保留时间定性,峰面积定量。结果一次进样分析仅需18min。该方法的线性范围为0~400μg/ml。苯甲酸的相关系数r=0.998 8,山梨酸钾的相关系数r=0.999 8,检出限0.001 7μg/ml,回收率99.65%,相对偏差1.3%。结论该法准确、快速、简便,可推广应用。Objective A HPLC method for the determina- tion of benzoic acid, potassium sorbate in beverage was established. Methods The sample was extracted and the components were separated on a C18 reversed phase column eluated with a mobile phase of methyl alcohol and ammonium acetate （5：95）. At the wave length of 230 nm, the 10 μl sample was detected by High Per- formance Liquid Chromatography.The flow velocity was 1.0 ml/min. The method was qualified by the ratio between the retention time of chroma- tographic peak of the samples and standard substance, which means qualified the location of the chromatograph- ic peak. And to quantify it through the area of chromatographic peak is proportional to the concentration. Re- suits Analysis of one injection only need 18 min. Linearity of the calibration curve wasin the range from 0 to 400 μg/ml. The correlation index of benzoic acid and potassium sorbate were 0. 998 8, 0. 999 8 respectively. The average recovery rate was 99. 65% and relative standard deviation （ n = 5 ） was 1.3%, while the concen- tration limit was 0. 001 7 μg/ml. Conclusion Thismethod is accurate, fast and simple, and is worth to be pop- ularized and applied.

关 键 词：高效液相色谱法 苯甲酸 山梨酸钾 

分 类 号：O657.7[理学—分析化学]