牙龈卟啉单胞菌唾液酸酶基因缺失影响其感染上皮细胞后炎症相关microRNA的表达  

Effects of Abrogation of Neuraminidase in Porphyromonas Gingivalis on Inflammation-related MicroRNA Expressions after Infected with Gingival Epithelial Cells

作　　者：李琛[1] 杨雪[1] 潘亚萍[1] 徐晓宇[1] 佟彤[1] 郭艳[2] 

机构地区：[1]中国医科大学附属口腔医院牙周病科,辽宁省口腔医学研究所牙周病学研究室,辽宁省口腔疾病转化医学研究中心,辽宁沈阳110002 [2]中国医科大学附属口腔医院中心实验室,辽宁省口腔疾病重点实验室,辽宁沈阳110002

出　　处：《口腔医学研究》2017年第2期132-134,共3页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:81200785);辽宁省自然科学基金(编号:2015010496-301)

摘　　要：目的:检测牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)唾液酸酶基因(PG0352)缺失是否影响其感染上皮细胞后对上皮细胞炎症相关小RNA的调控。方法:厌氧培养P.gingivalis W83及其唾液酸酶基因突变株(△PG0352),以MOI为100∶1的比例分别与永生化牙龈上皮细胞epi4进行共培养,PCR array的方法检测88个与炎症相关的小RNA的表达。结果:△PG0352组epi4的hsa-miR-9、hsa-miR-23a、hsa-miR-23b和hsa-miR-410的表达分别是P.gingivalis W83组的4.3387、3.3978、4.7930和4.3137倍。结论:与P.gingivalis W83野生株相比,唾液酸酶基因缺陷株对牙龈上皮细胞炎症相关小RNA的表达有不同的调控作用。Objective：To investigate effects of abrogation of neuraminidase in porphyromonas gingivalis on inflammation-related microRNA expressions after infected with gingival epithelial cells.Methods：P.gingivalis W83 and PG0352 mutant strain（△PG0352）were cultured anaerobically,and co-cultured with gingival epithelial cells at the MOI of 100：1.Inflammation-related microRNAs were detected by real-time PCR.Results：The expressions of hsa-miR-9,hsa-miR-23 a,hsa-miR-23 b,and hsa-miR-410 were significantly higher in△PG0352group than those in P.gingivalis W83 group.The fold changes were 4.3387,3.3978,4.7930 and 4.3137,respectively.Conclusion：Neuraminidase deficient strain showed different effects on inflammation-related microRNA expression in gingival epithelial cells compared with wild-type strain.

关 键 词：牙龈卟啉单胞菌W83 PG0352 牙龈上皮细胞 小RNA 

分 类 号：R781.4[医药卫生—口腔医学]