种植体穿龈部分氮化钛纳米涂层对牙龈成纤维细胞粘附和增殖的影响  

Effect of TiN Nano-coating on Adhesion and Proliferation of Human Gingival Fibroblasts in Transmucosal Part of Dental Implant

作　　者：潘夏薇[1] 赖颖真[1] 陈江[2] 

机构地区：[1]厦门医学高等专科学校,福建厦门361000 [2]福建医科大学附属口腔医院种植科,福建福州350000

出　　处：《口腔医学研究》2017年第2期154-157,共4页Journal of Oral Science Research

基　　金：福建省临床重点专科建设项目(编号:20121589)

摘　　要：目的:明确氮化钛纳米(TiN)涂层对人牙龈成纤维细胞(HGF)粘附形态和增殖的影响,作为选择合适的种植体穿龈部分涂层的依据之一。方法:利用磁控溅射技术制作氮化钛(TiN)和对照Ti涂层,测量各组材料表面形貌、元素含量、粗糙度和亲水性,原代培养人牙龈成纤维细胞(HGF),通过扫描电镜SEM和CCK-8观察分析不同纳米涂层上方细胞粘附和增殖差异。结果:TiN组表面HGF粘附形态与Ti组相似,而HGF增殖幅度大于对照Ti。结论:本实验所设计的氮化钛(TiN)涂层利于促进HGF增殖,推荐作为种植体穿龈部分涂层。Objective：To investigate the effect of TiN nano-coating on adhesion and proliferation of the human gingival fibroblasts（HGF）.Methods：TiN coatings were deposited by magnetron sputtering.Ti coatings were deposited as the controls.The morphology and roughness of nano-coatings,elementary composition and hydrophilia were determined.Morphology of HGF was analyzed by using SEM.Cell proliferation abilities were investigated by using CCK-8Kit.Results：Morphology of HGF was similar between TiN coatings and the control.However,the cell proliferation ability was significantly increased in substrate with TiN nano-coating.Conclusion：TiN nanocoating can be applied in transmucosal part of dental implant to enhance the biocompatibility.

关 键 词：软组织 种植体 氮化钛(TiN)纳米涂层 人牙龈成纤维细胞(HGF) 细胞增殖 

分 类 号：R783.6[医药卫生—口腔医学]