中国拟青霉产色素发酵条件的研究  

Study on the Producting Conditions of Pigment by Paecilomyces sinensis

作　　者：朱振元[1] 韩丹 庞伟 ZHU Zhen-yuan HAN Dan PANG Wei(Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, Chin)

机构地区：[1]食品营养与安全教育部重点实验室天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品研究与开发》2017年第2期190-195,共6页Food Research and Development

基　　金：国家星火计划重点项目(2015GA610001);天津市农业科技成果转化与推广项目(201502180)

摘　　要：以中国拟青霉液体发酵菌丝体为研究对象,研究利用生物发酵生产色素的最适培养条件,为解决天然色素的局限性开辟一条新的道路。首先研究了中国拟青霉产色素的最适碳源、氮源,结果显示,最适碳源和氮源分别为葡萄糖和蛋白胨。在此基础上,分别以温度、摇床转速、p H值这些环境因素为单一变量,结果得出:合成色素的最适合温度、p H值和转速分别为25℃、水作为缓冲液(中性条件下)和设定摇床转速为160 r/min。根据单因素研究结果进行响应面分析并与试验值进行对照,结果表明试验值的误差小于2%,与响应值吻合。The liquid fermentation condition for the Paecilomyces sinensis was optimized in this essay in order to expand the usage of natural pigment. Firstly,the most suitable carbon source and nitrogen source was selected to be glucose and peptone,respectively. Afterward,other conditions such as temperature,shaking speed,p H value were optimized by single factor experiment under the condition of using them as carbon and nitrogen source. The result showed that the optimum figure for temperature,p H and shaking speed were 25 ℃,neutral and 160 r/min,respectively. Furthermore,response surface analysis was conducted to verify the results. The optimum figures showed less than 2 % variance in comparing with the theoretical response value

关 键 词：色价 环境因素 中国拟青霉 最适发酵条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TS264.4