MBR+蠕虫反应器膜污染特征及微生物群落结构  

Analysis of membrane fouling and microbial community structure in an MBR+worm reactor

作　　者：刘嘉[1,2] 左薇[2] 张军[2] 李慧[2] 李俐频 田禹[1,2] 

机构地区：[1]城市水资源与水环境国家重点实验室(哈尔滨工业大学),哈尔滨150090 [2]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《哈尔滨工业大学学报》2017年第2期32-36,共5页Journal of Harbin Institute of Technology

基　　金：水体污染控制与治理科技重大专项(2013ZX07201007);黑龙江省杰出青年科学基金(JC201303);哈尔滨工业大学城市水资源与水环境国家重点实验室项目(2014DX03);哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金(QA201207)

摘　　要：为研究针对MBR+蠕虫反应器工艺中蠕虫捕食对膜污染的影响,在常温下分别平行运行MBR+蠕虫反应器(R1)和作为对照系统的MBR+空白蠕虫反应器(R2).监测R1工艺中MBR(S-MBR)和R2工艺中MBR(C-MBR)的跨膜压力(pTM),检测污泥混合液及泥饼层微生物代谢产物的变化.利用变性梯度凝胶电泳(DGGE)技术分析S-MBR和C-MBR中微生物种类和分布.结果表明:S-MBR的膜污染周期为90 d,C-MBR的膜污染周期为28~37 d,蠕虫捕食导致S-MBR中SMP和EPS的多糖和蛋白质减少.S-MBR膜丝表面是微生物菌群Alphaproteobacterium,Betaproteobacterium,Deltaproteobacterium和Geobacter,而C-MBR膜丝表面是微生物菌群Azorhizobium,Rhodobacter,Gammaproteobacterium和Flavobacteria,对MBR膜污染进程起主要作用.Caldilinea可能与S-MBR膜污染减轻有关.蠕虫捕食可改变微生物群落结构,减缓S-MBR膜污染.To study the effects of worm predation on membrane fouling in an MBR＋worm reactor,an MBR＋worm reactor with worm（ R1） and an MBR ＋ worm reactor without worm（ R2） were operated in parallel. Variation of transmembrane pressure（ pTM） and microbial metabolites were studied in the MBR（ S-MBR） of the R1 and the MBR（ C-MBR） of the R2. Denatured gradient gel electrophoresis（ DGGE） was used for analyzing the composition and distribution of microbial community in the S- MBR and the C- MBR. The results showed that the membrane fouling cycles of the S-MBR and the C-MBR were 90 d and 28-37 d,respectively. Worm predation decreased the polysaccharide and proteins in the soluble microbial products（ SMP） and extracellular polymeric substances（ EPS）of the S-MBR. Alphaproteobacterium,Betaproteobacterium,Deltaproteobacterium,Geobacter on the membrane wire surface of the S- MBR and Azorhizobium,Rhodobacter,Gammaproteobacterium,Flavobacteria on the membrane wire surface of the C- MBR played an important role in the membrane fouling. Caldilinea was suggested to be related to the membrane fouling alleviation of the S- MBR. Worm predation changed the microbial community structure of the S-MBR,resulting in membrane fouling alleviation.

关 键 词：膜生物反应器 膜污染 胞外聚合物 溶解性微生物代谢产物 微生物群落结构 

分 类 号：X172[环境科学与工程—环境科学]