藤梨根提取物对人胃癌SGC7901细胞增殖抑制的影响  

Effect of Rattan pear root extract on SGC7901 gastric cancer cell growth by intervening cell cycle and inducing apoptosis

作　　者：党辉[1] 牟宗玲[2] 宋锐[2] 王涛[1] 关徐涛[1] 高萍[1] 

机构地区：[1]河南中医药大学第一附属医院,河南郑州450000 [2]山东体育学院

出　　处：《中国老年学杂志》2017年第4期786-788,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81301726);河南省教育厅自然科学基础研究计划(2010A360003)

摘　　要：目的研究藤梨根提取物对胃癌细胞SGC7901的抑制作用及其机制。方法用MTT法检测不同剂量的藤梨根提取物对人胃癌细胞SGC7901的增殖抑制作用,并用流式细胞仪分别检测细胞周期以及凋亡程度。结果在浓度范围25~200μg/ml范围内,不同浓度的藤梨根提取物对胃癌细胞SGC7901均有抑制作用,且有一定的浓度依赖性。且在藤梨根提取物细胞浓度为53、86以及142μg/ml作用48 h后SGC7901在S期比例增多,凋亡率明显增加。结论藤梨根提取物可能通过干预细胞周期和凋亡对胃癌细胞SGC7901增殖起抑制作用,同时与药物浓度有一定的依赖性。Objective To observe the inhibitory effect of Rattan pear root extract on gastric cancer SGC7901 cells and its mechanism. Methods MTT was used to detect different doses of Rattan pear root extract inhibition on proliferation of human gastric cancer SGC7901 cells. Cell cycle and apoptosis were detected.Results In the concentration range from 25 to 200 μg / ml,different concentrations of Rattan pear root extract had inhibitory effect on gastric cancer cells SGC7901 with concentration dependence. Treated with 53,86 μg / ml and 142 μg / ml Rattan pear root extract for 48 h,the SGC7901 in S phase ratio was increased,the apoptosis rate was increased significantly.Conclusions Rattan pear root extract might inhibit gastric cancer SGC7901 cells proliferation by intervening in the cell cycle and apoptosis,at the same time drug concentration has certain dependence.

关 键 词：藤梨根提取物 胃癌细胞SGC7901 细胞凋亡 细胞周期 

分 类 号：R73[医药卫生—肿瘤]