PDZK1基因过表达对宫颈癌HeLa细胞生物学行为的作用  

Effect of PDZK1 overexpression on the biological behaviors of cervical carcinoma HeLa cells

作　　者：于洋 高聪 红霞 张妮娜 

机构地区：[1]内蒙古兴安盟人民医院妇二科,内蒙古乌兰浩特137400

出　　处：《武警后勤学院学报（医学版）》2016年第12期969-973,共5页Journal of Logistics University of PAP（Medical Sciences）

摘　　要：【目的】通过上调PDZ结构域蛋白1(PDZ domain containing 1,PDZK1)基因表达,探讨PDZK1基因对宫颈癌HeLa细胞生物学行为的调控作用。【方法】将PDZK1表达质粒和空载体质粒分别转染HeLa宫颈癌细胞,经G418筛选及Western印迹验证,获得整合了PDZK1及空载体的稳转HeLa细胞。CCK-8法检测两种细胞增殖活性、软琼脂集落形成实验检测细胞锚定非依赖生长能力、Annexin V-FITC/PI双染流式细胞术检测细胞凋亡率、Transwell迁移实验检测细胞迁移能力。【结果】与整合空载体的HeLa细胞相比,PDZK1过表达可显著抑制HeLa细胞增殖能力(第5天P=0.001,第6、7天P<0.001),同时降低HeLa细胞锚定非依赖生长能力[(1.67±0.58)和(3.67±0.58)],差异有统计学意义(t=4.243,P=0.013)。相比整合了空载体的HeLa细胞,PDZK1过表达可以促进HeLa细胞凋亡[(4.70±0.56)%和(1.40±0.10)%],差异有统计学意义(t=10.014,P=0.001)。此外,整合PDZK1的HeLa细胞其迁移能力(21.33±4.16)显著低于整合空载体的HeLa细胞(52.33±6.02),差异有统计学意义(t=-7.392,P=0.002)。【结论】上调PDZK1基因表达可以抑制宫颈癌HeLa细胞增殖和迁移,并促进其凋亡。【Objective】To explore the effect of upregulating PDZK1 expression level on the biological behaviors of cervical carcinoma cell line HeLa.【Methods】PDZK1-expressing plasmid and empty vector were constructed and transfected into cervical carcinoma HeLa cells. After G418 screening, the PDZK1-transfected HeLa cells were obtained and verified by Western blot analysis. The proliferation ability of these two kinds of cells were detected by CCK-8 method, and anchorage-independent growth ability was determined by colony formation in soft agar. Annexin V-fluorescein isothiocyanate（FITC）/propidium iodide（PI） flow cytometry was used to detect the rate of apoptosis. Transwell assay was used to detect the ability of migration.【Results】Compared with empty vector-transfected control cells,PDZK1 overexpression could significantly inhibit the proliferation ability of HeLa cells（P=0.001 on day5, P〈0.001 on day6 and day7）,and the ability of anchorage-independent growth [（1.67±0.58） and（3.67±0.58）]（t=4.243, P=0.013）. Compared with empty vectortransfected control cells, the apoptosis rate of PDZK1 overexpression was significantly higher [（4.70±0.56）% and（1.40±0.10）]（t=10.014, P=0.001）. The migration ability of PDZK1-transfected HeLa cells（21.33±4.16） was significantly lower than that of empty vector-transfected control cells（52.33±6.02）（t=-7.392, P=0.002）【Conclusion】Up-regulating the expression level of PDZK1 can inhibit the proliferation and migration of cervical carcinoma HeLa cells, as well as promote the apoptosis.

关 键 词：宫颈癌 PDZ结构域蛋白1 集落形成能力 细胞迁移 

分 类 号：R737.33[医药卫生—肿瘤]