帕金森病患者唾液α-突触核蛋白与纹状体多巴胺摄取功能的相关性研究  

The association of salivary α-synudein concentration and nigrostriatal dopaminergic function in Parkinson＇ s disease

作　　者：张佳[1] 康文岩[1] 刘军[1] Zhang Jia Kang Wenyan Liu Jun.(Department of Neurology ＆ Institute of Neurology, Ruijin Hospital Affiliated To Shanghai Jiaotong University School of Medicine, Shanghai 200025, Chin)

机构地区：[1]上海交通大学医学院附属瑞金医院神经内科,200025

出　　处：《临床内科杂志》2017年第1期45-48,共4页Journal of Clinical Internal Medicine

摘　　要：目的分析帕金森病（PD）患者唾液中的α-突触核蛋白含量与纹状体多巴胺摄取功能的相关性，探讨其是否可能作为PD的早期生物学标志物。方法选取门诊确诊的原发性PD患者70例，根据发病年龄分为早发组（EOPD组，发病年龄≤50岁）25例和晚发组（LOPD组，发病年龄〉50岁）45例。应用Luminex技术检测并比较两组PD患者唾液α-突触核蛋白的水平，运用放射性核素^99m锝一巯胺托品标记的单光子发射计算机断层扫描（^99mTc-TRODAT-1 SPECT）检测PD患者脑内纹状体多巴胺摄取功能，并分析PD患者唾液α-突触核蛋白含量与纹状体多巴胺摄取功能的相关性。结果LOPD组患者唾液中α-突触核蛋白相对平均含量较EOPD组明显降低（t=2．833，P=0．005）。总体70例PD患者唾液中α-突触核蛋白含量与纹状体、壳核、尾状核多巴胺摄取功能均无相关性（P〉0．05）；性别、年龄校正前，EOPD组患者唾液中α-突触核蛋白含量与壳核^99mTc—TRODAT-1摄取功能呈较弱的正相关（r=0．4082，P=0．0428），校正后无相关性（r=0．1329，P=0．5454）；LOPD组唾液中α-突触核蛋白含量与纹状体壳核及尾状核^99mTc—TRODAT-1摄取功能均无相关性（P〉0．05）。结论PD患者唾液中α-突触核蛋白含量随年龄增加而下降，其与纹状体多巴胺摄取功能无明显相关性。Objective To explore whether salivary α-synuclein could be a potential biomarker for monitoring disease progression in Parkinson＇ s disease （PD） by evaluating the association between salivary α-synuclein concentration and nigrostriatal dopaminergic function. Methods Seventy consecutive outpatient clinic PD cases were recruited, of which were divided into early-onset PD group （ EOPD group, n = 14, age at onset ≤50 years old） and late-onset group （LOPD group, n = 56, age at onset 〉 50 years old）. Firstly, saliva of PD patients were collected and α-synuclein levels in saliva were analyzed by quantitative and sensitive Luminex assay. Then, single photon emission computed tomography（SPECT） imaging with labeled dopamine transporters（DAT） （^99mTc-TRODAT-1） ,which has been used for measuring DAT density in PD was performed. Finally, we studied the relationship between saliva α-synuclein levels and nigrostriatal dopaminergic function. Results The salivary α-synuclein was significantly lower in LOPD group compared to EOPD group（ t = 2. 833 ,P = 0. 005 ）. No correlation was found between salivary a-synuclein levels and nigrostriatal uptake of ^99mTc-TRODAT-1 in total PD and LOPD patients, no matter controlled for age and gender or not （P 〉 0. 05 ）. There was a slight correlation between salivary α-synuclein levels and putamen nucleus uptake of ^99mTc-TRODAT-1 in EOPD patients before adjustment for age and gender（r = 0. 4082, P = 0. 0428）, but was no correlation after adjustment for age and gender （ r = 0. 1329, P = 0, 5454 ）. Conclusion Salivary α-synuelein level decreases with age but has no correlation with nigrostriatal dopaminergic funetion.

关 键 词：Α-突触核蛋白 唾液生物学标志物 帕金森病 多巴胺转运体-单光子发射计算机断层扫描 

分 类 号：R742.5[医药卫生—神经病学与精神病学]