小鹅瘟病毒和鹅沙门氏菌PCR-DHPLC检测方法  

Development and Preliminary Application of PCR-DHPLC for Goose Parvovirus and Salmonella

作　　者：许尧[1,2] 董航[3] 张兴菊 毛文智 邵洪泽[3] 胡桂学[1] XU Yao DONG Hang ZHANG Xing-ju MAO Wen-zhi SHAO Hong-ze HU Gui-xue(Jilin Agricultural University, Jilin, 130118, Changchun, Jilin Jilin Animal Disease Prevention and Control Center, Changchun, Jilin130062 Jinlin Veterinary Science Institute, Changchun, Ji lin130062 Animal Husbandry Station of Jilin Province, Changchun, Jlin 130062 Jiutai Animal Disease Prevention and Control Center, Changchun, Jilin 130500)

机构地区：[1]吉林农业大学,吉林长春130118 [2]吉林省动物疾病预防控制中心,吉林长春130062 [3]吉林省畜牧兽医科学研究院,吉林长春130062 [4]吉林省长春市九台区动物疫病预防控制中心,吉林长春130500 [5]吉林省畜牧总站,吉林长春130062

出　　处：《吉林畜牧兽医》2017年第2期8-11,共4页Jilin Animal Husbandry and Veterinary Medicine

基　　金：吉林省科技发展计划资助项目,编号20140204075NY

摘　　要：小鹅瘟和沙门氏菌感染是严重危害养鹅业发展的常见病,为了快速、准确地对小鹅瘟、鹅沙门氏菌感染进行诊断,减少这两种病的危害,本试验将这两种病原体的PCR-DHPLC检测方法结合起来,建立一种可以同时检测小鹅瘟和鹅沙门氏菌的方法,并进行了初步应用。结果表明这种新的PCR-DHPLC方法稳定、敏感、特异性强,适合专业检测使用。In order to quickly and accurately diagnose 6oose Parvovirus and Salmonella, reduce the hazard of these goose diseases, this study combined these two PCR-DHPLC detection methods of pathogens, to establish a method of detecting both Goose Parvovirus and Salmonella and preliminary applicationo The results showed that this new PCR DHPLC method is stable, sensitive, specific and suitable for professional use.

关 键 词：小鹅瘟 沙门氏菌 PCR—DHPLC 检测 

分 类 号：S858.315.1[农业科学—临床兽医学]