野生猕猴桃根黄酮提取物对胃癌MKN-49P细胞增殖影响  

Effect of wild Chinese actinidia root flavonoids extracts on gastric cancer MKN-49P cell proliferation

作　　者：杨晓丹[1] 韩涛[1] 谢晓冬[1] 刘兆喆[1] 郑振东[1] 

机构地区：[1]沈阳军区总医院肿瘤科,辽宁沈阳110016

出　　处：《临床军医杂志》2017年第1期27-29,共3页Clinical Journal of Medical Officers

基　　金：辽宁省博士科研启动基金(201501022);军队医学科技青年培育计划(15QNP005);北京医卫健康公益基金(YWKYQ2001)

摘　　要：目的探讨野生猕猴桃根黄酮提取物对胃癌MKN-49P细胞增殖作用的影响及机制。方法采用CCK-8比色法、流式细胞术、Western Blot法检测野生猕猴桃根黄酮提取物对胃癌MKN-49P细胞增殖作用的影响。结果野生猕猴桃根黄酮提取物对胃癌MKN-49P细胞增殖有明显的抑制作用,且呈时间-浓度依赖性;其浓度升高,细胞凋亡增加,G1期细胞增加,S、G2/M期细胞减少,抑制周期蛋白CyclinD1表达降低。结论野生猕猴桃根黄酮提取物可抑制胃癌MKN-49P细胞增殖、促进细胞凋亡、阻滞细胞周期于G1期,其主要机制可能与抑制周期蛋白CyclinD1表达降低有关。Objective To investigate the effect and mechanism of wild Chinese actinidia root flavonoids extracts in gastric cancer MKN-49 P cell proliferation. Methods Using the CCK-8 assay,flow cytometric（ FCM） analysis and Western Blot method to detect the effect of flavonoids on gastric cancer MKN-49 P cell proliferation. Results Wild Chinese actinidia root flavonoids extracts had obvious inhibitory on gastric cancer MKN-49 P cell proliferation in a time-concentration dependence; as concentration increased,apoptosis increased,G1 staging cells increased,S and G2 / M staging cells reduced,protein expression of Cyclin D1 which inhibiting cell cycle reduced. Conclusion Wild Chinese actinidia root flavonoids extracts can inhibit gastric cancer MKN-49 P cell proliferation,promote cells apoptosis,inhibit cell cycle in G1 staging,the main mechanism is probably related with protein expression of Gyclin D1 which inhibiting cell cycle reduced.

关 键 词：野生猕猴桃根黄酮提取物 胃癌MKN-49P细胞 增殖 凋亡 细胞周期 

分 类 号：R730.5[医药卫生—肿瘤]