载有人胚胎干细胞的多孔磷酸钙骨水泥与富血小板血浆复合后修复大鼠骨缺损的实验研究  

Macroporous calcium phosphate cement, human embryonic stem cells and platelet-rich plasma constructs for bone regeneration in cranial defects in rats

作　　者：吴珍珍[1] 包崇云[1] 李明政[1] 刘显[1] 

机构地区：[1]四川大学,口腔疾病研究国家重点实验室,华西口腔医院,四川成都610041

出　　处：《中国口腔颌面外科杂志》2017年第1期6-10,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金青年基金(31400829)

摘　　要：目的 :研究富血小板血浆(platelet rich plasma,PRP)对负载人胚胎来源的间充质干细胞(h ESC-MSCs)多孔磷酸钙骨水泥(calcium phosphate cements,CPC)复合物的成骨促进作用。方法:将h ESC-MSCs接种于CPC表面,扫描电镜(SEM)观察h ESC-MSCs在CPC表面的黏附情况。建立直径为8 mm的大鼠颅骨圆形缺损模型,将18只大鼠随机分为3组,分别是CPC+h ESC-MSCs组、CPC+h ESC-MSCs+30%PRP组和CPC+h ESC-MSCs+50%PRP组。术后12周显微CT和H-E染色观察新骨和血管生成情况。应用SPSS16.0软件包对数据进行单因素方差分析。结果:SEM显示,细胞可良好地黏附在CPC支架的表面。显微CT示复合PRP后,CPC材料的降解程度逐渐明显,且新生骨量逐渐增加。H-E染色显示,复合PRP组的新骨生成率和血管密度高于未复合PRP组,但是复合不同浓度的PRP组间无显著差异。结论:浓度为30%和50%的PRP与负载h ESC-MSCs的CPC复合后,均可促进骨缺损区新骨的生成。PURPOSE： To investigate the effects of macroporous calcium phosphate cement（CPC）, human embryonic stem cells（h ESC） and platelet-rich plasma constructs for bone regeneration in vivo in critical-sized cranial defects in rats.METHODS： Three groups were tested in rats：（a） CPC with h ESC-derived mesenchymal stem cells（CPC＋h ESC-MSCs）;（b） CPC with h ESC-MSCs and 30% platelet rich plasma（PRP）（CPC＋h ESC-MSCs＋30%PRP） and（c） CPC＋h ESC-MSCs＋50%PRP. 18 rats were sacrificed 12 weeks after operation. Micro-CT and bone histomorphologic analysis were conducted to compare the status of new bone formation. SPSS16.0 software package was used for one way analysis of variance.RESULTS： At 12 weeks in vivo, the groups with h ESC-MSCs and PRP had more new bone formation compared with other two groups; however, there was no significant difference in new bone area fraction between different concentration of PRP groups. CONCLUSION： Macroporous CPC with h ESC-MSCs and PRP may be useful for bone regeneration in craniofacial and orthopedic fields.

关 键 词：磷酸钙骨水泥 人胚胎干细胞 富血小板血浆 骨组织工程 

分 类 号：R782[医药卫生—口腔医学]