维生素D受体基因Apa1、Taq1多态性与肺鳞癌易感性的关系  

Association of vitamin D receptor gene Apa1 and Taq1 polymorphisms with susceptibility to lung squamous cell carcinoma

作　　者：杨雁鸿[1] 吴剑冬[1] 周文文[1] 侯燕燕[1] 尹端端[1] 王真真[2] 

机构地区：[1]河北省秦皇岛市第一医院肿瘤科,河北秦皇岛066000 [2]承德医学院研究生院,河北承德067000

出　　处：《河北医科大学学报》2017年第1期20-23,71,共5页Journal of Hebei Medical University

基　　金：秦皇岛市科学技术研究与发展计划(201101A259)

摘　　要：目的探讨维生素D受体(vitamin D receptor,VDR)Apa1、Taq1两基因位点的多态性与肺鳞癌易感性之间的关系。方法采集288例肺鳞癌患者和284例健康对照个体肘静脉血5 m L,采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术方法,对VDR Apa1、Taq1两基因位点行基因型检测。结果 VDR Taq1位点基因多态性是肺鳞癌发生的危险因素,其中tt+Tt基因型携带者发生肺鳞癌的风险是TT基因型携带者的0.556倍(OR=0.556,95%CI=0.393~0.785)。按是否吸烟分层分析:吸烟人群中,Taq1位点基因多态性在病例组与对照组间差异有统计学意义,tt+Tt基因型携带者发生肺鳞癌的风险是TT基因型携带者的0.049倍(OR=0.049,95%CI=0.259~0.929);t等位基因携带者发生肺鳞癌的风险是T等位基因携带者的0.444倍(OR=0.444,95%CI=0.241~0.817)。而VDR Apa1位点多态性与肺鳞癌易感性无相关性。结论 VDR Taq1基因多态性与肺鳞癌的遗传易感性相关,tt+Tt基因携带者肺鳞癌发病风险降低;t等位基因与患肺鳞癌风险呈负相关;吸烟者中tt+Tt基因携带者肺鳞癌的发病风险显著降低。Objective To investigate the relationship between polymorphism of vitamin D receptor（VDR） gene（including Apal, Taql） and susceptibility of squamous cell carcinoma of lung cancer（SCC）. Methods The blood samples were collected from 288 patients with SCC and 284 healthy individuals. Leukocyte DNA from peripheral blood was extracted. The method of polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was applied to test the genotype of Apal, Taql in VDR gene. Results VDR Taql gene polymorphisms is the risk factor of SCC. The SCC risk of tt＋Tt genotype carriers is 0.556 times than the TT genotype carriers（OR=0.556, 95%CI=0.393-0.785）. Based on stratification analysis of smoking： In polymorphism is related to genetic susceptibility of SCC. The tt ＋ Tt gene reduces the risk of SCC. The t allele is negatively correlated with the risk of SCC. Smokers with tt＋Tt gene are significantly reduced the risk of SCC.

关 键 词：肺肿瘤 维生素D 基因 

分 类 号：R734.2[医药卫生—肿瘤]