1株异养反硝化硫细菌的分离鉴定及代谢特性  

Isolation,Identification and Metabolic Characteristics of a Heterotrophic Denitrifying Sulfur Bacterial Strain

作　　者：谭文勃[1] 马晓丹[1] 黄聪[1] 陈川[1] 王爱杰[1,2] 

机构地区：[1]哈尔滨工业大学市政环境工程学院城市水资源与水环境国家重点实验室,哈尔滨150090 [2]中国科学院生态环境研究中心环境生物技术重点实验室,北京100085

出　　处：《环境科学》2017年第2期809-814,共6页Environmental Science

基　　金：国家自然科学基金项目(51225802,51308147)

摘　　要：工业废水中的有机物、硫化物和含氮化合物是废水处理面临的重大挑战.利用生物技术,可以实现在废水处理系统中同时去除这3种污染物.在污水处理反应器中,分离出1株异养反硝化硫细菌HDD1.基于16S rRNA基因的系统进化分析和生理特征显示菌株HDD1为Thauera属的一个种.菌株HDD1能够利用乙酸盐和硫化物作为电子供体,硝酸盐作为电子受体进行呼吸作用.在15h之内,CH3COO-(300 mg·L^(-1))、S2-(200 mg·L^(-1))和NO-3(487 mg·L^(-1))被完全代谢去除.扫描电子显微镜和能量色散谱结果显示硫化物氧化的主要产物为单质硫.菌株HDD1能够同时应用于工业废水处理和硫元素的资源化回收.Organics,sulfide and nitrogen compounds in industrial wastewater are significant challenges for wastewater treatment. These pollutants could be removed simultaneously from wastewater treatment system using biological technologies. In this study, a heterotrophic denitrifying sulfur bacterial strain HDD1 was isolated from wastewater treatment bioreactor. Strain HDD1 was identified as Thauera sp. based on the 16S rRNA gene phylogenetic analysis and physiological characteristics. Acetate and sulfide could be utilized as electron donors and nitrate as electron acceptor for respiration in Thauera sp. HDD1. The acetate （300 mg·L ^- 1 ）, sulfide （200 mg·L ^- 1 ） and nitrate （487 mg·L ^- 1 ） were completely metabolized and removed within 15 hours. The main product of sulfide oxidation was elemental sulfur as identified by scanning electron microscope and energy dispersive spectrometer. These results suggest that the newly isolated Thauera sp. HDD1 could be used for simultaneous industrial wastewater treatment and elemental sulfur resource recovery.

关 键 词：硫化物 单质硫 乙酸盐 硝酸盐 反硝化作用 

分 类 号：X172[环境科学与工程—环境科学]