基因芯片法分析MTHFR基因C677T还原酶位点多态性与原因不明不良孕产的关系  

Analysis on relationship between MTHFR C677T locus polymorphism and unexplained adverse pregnancy by gene chip method

作　　者：孙政敏[1] 王华[1] 冯东[1] 

机构地区：[1]河南省新乡市第一人民医院/新乡医学院附属人民医院检验科,453000

出　　处：《国际检验医学杂志》2017年第4期473-474,477,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨5,10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点多态性与原因不明不良孕产的关系。方法选取2014年6月至2016年5月在新乡市第一人民医院不孕不育科、妇产科就诊的原因不明不良孕产≥2次320例孕妇作为病例组,388例无流产史健康经产妇作为对照组。采用基因芯片法分析两组人群MTHFR基因C677T位点多态性;比较组间基因型、等位基因频率分布的差异,分析原因不明不良孕产与MTHFR C677T位点多态性的关系。结果 MTHFR C677T基因型C/C频率分布病例组和对照组比较差异有统计学意义(P<0.05、OR=0.284);基因型C/T频率分布两组间比较差异无统计学意义(P=0.400、OR=1.140);基因型T/T频率分布两组间比较差异有统计学意义(P<0.05、OR=7.672);等位基因C、T频率分布两组间比较差异有统计学意义(P<0.05、OR=0.304)。结论 MTHFR C677T基因型T/T的高表达可能是育龄女性妇女原因不明不良孕产的危险因素。Objective To investigate the relationship between 5,10-methylenetetrahydrofolate reductase（MTHFR）gene C677 T locus polymorphism and unexplained adverse pregnancy.Methods Three hundreds and twenty pregnant women with unknown adverse pregnancy≥2times in the infertility department,and obstetrics and gynecology department of our hospital from June 2014 to May 2016 were selected as the case group and 388 healthy non-abortion parous women were taken as the control group.The MTHFR gene C677 Tlocus polymorphism in the two groups was analyzed by using gene chip method.The differences in genotype and allele frequency distribution were compared between the two groups,and the relationship between unexplained adverse pregnancy and MTHFR C677 Tlocus polymorphism was analyzed.Results The frequency distribution of MTHFR genotype C677TC/C had statistically significant difference between the case group and control group（P〈0.05,OR=0.284）.The frequency distribution of genotype C/T had no statistical difference between the case group and control group（P=0.400,OR=1.140）.The frequency distribution of genotype T/T had statistical difference between the case group and control group（P〈0.05,OR=7.672）.The frequency distribution of allele C and T had statistical difference between the case group and control group（P〈0.05,OR=0.304）.Conclusion The high expression of MTHFR C677 Tgenotype T/T may be a risk factor of unknown adverse pregnancy in childbearing age women.

关 键 词：原因不明 不良孕产 等位基因 

分 类 号：R714.21[医药卫生—妇产科学]