肝癌细胞中DNAJC6过表达可诱导上皮-间质转变过程的发生  

Overexpression of DNAJC6 induces the occurrence of EMT in hepatocellular carcinoma cells

作　　者：耿蕴峰 李晓娜 卢玉娟[1] 贾金广 杨涛[1] 

机构地区：[1]河北省石家庄市第一医院肝胆外科,河北石家庄050011 [2]河北省体育科学研究所门诊部,河北石家庄050011

出　　处：《河北医科大学学报》2017年第2期151-153,158,共4页Journal of Hebei Medical University

基　　金：河北省科技计划项目(16277705D);石家庄市科学技术研究与发展指导计划(141463243)

摘　　要：目的通过体外实验,探讨DNAJC6在肝细胞肝癌进展中的作用途径。方法应用体外肝癌细胞培养、质粒构建、转染及逆转录-聚合酶链反应检测肝癌细胞Hep G2中DNAJC6表达对上皮-间质转变(epithelial-tomesenchymal transition,EMT)过程的影响。结果与对照组比较,转染DNAJC6后的肝癌细胞Hep G2中EMT相关转录因子Twist1、Snail、Slug、ZEB1表达水平明显升高(P<0.01),间质细胞标记物神经-钙黏素(N-cadherin)和波形蛋白(Vimentin)表达上调(P<0.01),上皮标记物上皮-钙黏素(E-cadherin)和上皮细胞紧密连接蛋白(ZO-1)表达下调(P<0.01)。结论 DNAJC6通过诱导EMT过程参与肝细胞肝癌的进程。Objective To investigate the role of DNAJC6 in the progression of hepatocellular carcinoma in vitro. Methods The effects of DNAJC6 expression on epithelial-to-mesenchymal transition（EMT） in Hep G2 cells were detected by in vitro liver cancer cell culture,plasmid construction,transfection and reverse transcription-polymerize chain reaction. Results Compared with the control group,the expression level of EMT related transcription factor in hepatocellular carcinoma cell line Hep G2 transfected with DNAJC6 Twist1, Snail, Slug, ZEB1 was increased significantly（ P〈0. 01）.Mesenchymal cell markers N-cadherin and vimentin were up-regulated（ P〈0. 01）. Epithelial markers Ecadherin and ZO-1 were down-regulated（ P〈0. 01）. Conclusion DNAJC6 is involved in the process of hepatocellular carcinoma by inducing EMT.

关 键 词：肝肿瘤 转染 上皮-间质转变 

分 类 号：R575.2[医药卫生—消化系统]