参芪复方对糖尿病大鼠血管PGC-1 mRNA的影响  

Influence of Shenqi Compound Prescription on PGC-1 mRNA in the Blood Vessels of Rats with Diabetes

作　　者：岳宗相[1] 王艳红[1] 黄荣利[1] 谢春光[2] 钟振东[3] 

机构地区：[1]眉山市中医医院,四川眉山612160 [2]成都中医药大学,四川成都610075 [3]四川省人民医院,四川成都610070

出　　处：《河南中医》2017年第1期68-71,共4页Henan Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:30772806)

摘　　要：目的:观察参芪复方对糖尿病GK大鼠血管内质网调节因子转录辅助活化因子mRNA(PGC^(-1) mRNA)的影响。方法:取5月龄雄性SPF级GK大鼠55只,随机分为空白组14只、模型组13只、中药组14只和西药组14只,有1只Wistar大鼠血糖>6.7 mmol·L^(-1)予以剔除,把剩余的14只纳入到正常组。正常组、空白组及模型组大鼠灌服生理盐水5 m L·(kg·d)^(-1),每天1次,连续给药35 d;西药组大鼠灌服阿托伐他汀钙混悬液1.6 mg·(kg·d)^(-1),每天1次,连续给药35 d;中药组大鼠灌服参芪复方混悬液1.44 g·(kg·d)^(-1),每天1次,连续给药35 d。取大鼠腹主动脉,PCR技术检测血管组织PGC^(-1) mRNA。结果:与各组大鼠比较,中药组大鼠血管PGC^(-1) mRNA明显升高,差异具有统计学意义(P<0.05)。结论:参芪复方可以调节糖尿病大鼠血管内质网PGC^(-1) mRNA水平。Objective ：To observe the influence of Shenqi Compound Prescription on PGC-I mRNA in the blood vessels of rats with diabetes. Methods：Totally 55 male GKrats of grade SPF and 5 months old were randomized into blank group （ 14 cases） , model group（ 13 cases） ,Chinese medicine group （lg cases） and western medicine group （14 cases）. Among them,one Wistar rat had blood glucose 〉6.7 mmol · L-l and was excluded. The rest 14 rats were included into the normal group. The rats in normal group,blank group and model group were drenched normal saline 5 mg·（kg·d）（-1） ,once a day and for 35 days＇ successive ad- ministration. Western medicine rats were administered With atorvastatin suspension of 1.6 mg·（kg·d）（-1）, once every day and for 35 days＇ successive administration. Chinese medicine group were given suspension of Shenqi Compound Prescription, once ev- ery day and for 35 days＇ successive administration. The abdominal aortas were taken and the PGC-1 mRNA was detected by PCR. Results：Among all groups of rats,the level of PGC-1 mRNA in the rats＇ blood vessel in Chinese medicine group increased obvi- ously ;the difference had statistical significance （P 〈 0.05 ）. Conclusion：Shenqi Compound Prescription can regulate the level of PGC-1 mRNA in endoplasmic reticulum of the blood vessels in rats with diabetes.

关 键 词：参芪复方 糖尿病血管病变 PGC-1 MRNA GK大鼠 

分 类 号：R285.5[医药卫生—中药学]