miR-382对胃癌细胞系MGC-803迁移、侵袭和增殖的影响  

Effects of miR-382 on cell migration, invasion and proliferation of gastric cancer cell lines MGC-803

作　　者：汪毅[1] 步鹏[3] 李峰[2] 刘晓玲[5] 徐钧[4] 

机构地区：[1]国家癌症中心中国医学科学院北京协和医学院肿瘤医院VIP病房,北京100021 [2]山西省肿瘤医院分子生物室 [3]山西省肿瘤医院病理科 [4]山西省肿瘤医院外科 [5]中国医学科学院北京协和医学院基础学院生物化学系

出　　处：《中华医学杂志》2017年第8期612-615,共4页National Medical Journal of China

摘　　要：目的 探讨miR-382对胃癌细胞系MGC-803迁移、侵袭和增殖的影响.方法 实时（Real-time）聚合酶链反应（PCR）分析50例胃癌组织（C）及其癌旁正常组织（N）中miR-382的表达水平;用pcDNA3.1载体构建过表达miR-382的重组质粒（pcDNA-miR-382）,实现miR-382在MGC-803细胞中的过表达;分别用划痕实验、Transwell和CCK-8法检测细胞迁移、侵袭和增殖能力.结果 与癌旁对照组相比,41例（82％）胃癌组织中出现miR-382的表达下调;过表达miR-382能显著抑制MGC-803细胞的迁移、侵袭和增殖能力（P＜0.05）.结论 miR-382的表达下调可能与胃癌的进展有关,过表达miR-382可以抑制胃癌MGC-803细胞的迁移、侵袭和增殖.Objective To investigate the effects of miR-382 on cell migration,invasion and proliferation of gastric cancer cell lines MGC-803.Methods The level of miR-382 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues.miR-382 overexpression was achieved by transfection of construct pcDNA-miR-382 into MGC-803 cells.The migration,invasion and proliferation of MGC-803 cells were detected by the scratch wound assay,Transwell and CCK-8,respectively.Results miR-382 was decreased in 41 cases （82%） of gastric cancer tissues compared to their control.Furthermore,overexpression of miR-382 effectively inhibited the migration,invasion and proliferation of MGC-803 cells （P 〈 0.05）.Conclusion Down-regulation of miR-382 has a correlation with the progression of gastric cancer.Up-regulation of miR-382 can inhibit the migration,invasion and proliferation of MGC-803 cells.

关 键 词：胃肿瘤 miR-382 迁移 侵袭 增殖 

分 类 号：R735.2[医药卫生—肿瘤]