微小RNA-28-3p对骨巨细胞瘤生物学行为的影响  

Influence of microRNA -28 -3p on biological behariors of giant cell tumors of bone

作　　者：苗润生[1] 蔡林[1] 勘武生[2] 唐欢[2] 李鹏[2] 李鲲[2] 丁凡[2] 刘伟军[2] 赵志刚[2] 胡锐[2] 刘明[2] 张青松[2] 

机构地区：[1]武汉大学中南医院骨四科,430071 [2]武汉市普爱医院骨科,430034

出　　处：《中华实验外科杂志》2017年第2期261-263,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA（miRNA，miR）-28-3p在骨巨细胞瘤中的表达及其对骨巨细胞瘤生物学行为的影响。 方法收取20例肿瘤标本及瘤旁正常组织，利用Taqman荧光探针实时定量聚合酶链反应（Real-time PCR）技术和荧光原位杂交技术检测肿瘤组织及瘤旁正常组织中miR-28-3p的表达。以原代培养的骨巨细胞瘤基质细胞（GCTSC）为靶细胞转染miR-28-3p的仿生剂和抑制剂，通过镜下观察和噻唑蓝（MTT）实验检测miR-28-3p对GCTSC增殖活性的影响。 结果Real-time PCR检测骨巨细胞瘤中miR-28-3p的表达量显著低于瘤旁正常组织（0.98±0.11比3.27±0.65，P＝0.003）；荧光原位杂交显示肿瘤组织内荧光值明显低于瘤旁组织。GCTSC转染miR-28 agomir后，细胞增殖活性及细胞计数显著低于对照组（0.63±0.12比1.02±0.15，P＝0.024）；而转染miR-28 antagomir后，细胞增殖活性及细胞计数显著高于对照组（1.73±0.14比0.97±0.14，P＝0.002）。 结论miR-28-3p在骨巨细胞瘤中的表达量显著降低，在骨巨细胞瘤中起抑癌作用。Objective To determine the expression of microRNA （miRNA, miR） -28 -3p in gi- ant cell tumor of bone and its effect on the neoplasm biological behaviors. Methods The samples including tumor and adjacent normal tissues from 20 patients with giant cell tumor of bone were harvested. Real - time quantitative polymerase chain reaction （Real -time PCR） with Taqman probe technique and fluores- cence in situ hybridization were applied to ascertain miR -28 -3p expression in tumor tissue and adjacent normal tissues. Primary bone giant cell tumor stromal cells （GCTSCs） were used as target ceils were trans- fected with miR-28 -3p bionic agent and inhibitor, and microscopic observation and methyl thiazol tet- razolium （MTT） test were applied to evaluate the influence of miR - 28 - 3p on proliferative activity of GCTSCs. Results The real - time PCR tests showed that miR - 28 - 3p expression in tumor tissue was sig- nificantly lower than that in peri - tumorous normal tissues （ 0. 98 ± 0. 11 vs. 3.27 ± 0. 65, P = 0. 003 ）. Fluorescence in situ hybridization showed lower fluorescence value inside tumor than that in adjacent normal tissues. After transfection of GCTSCs with miR - 28 agomir, the cell proliferation activity and the cell counts were significantly reduced as compared with those in control group （0. 63 ±0. 12 vs. 1.02 ±0. 14, P = 0. 024）. On the contrary, after transfection GCTSCs with miR - 28 antagomir, the cell proliferation ac- tivity and the cell counts were significantly increased as compared with those in control group （ 1.73 ± 0. 14 vs. 0. 97 ± 0. 14, P = 0. 002）. Conclusion MiR - 28 - 3p is down - regulated in bone giant cell tumor, suggesting its antitumor effect.

关 键 词：微小RNA-28-3p 骨巨细胞瘤 基因表达 

分 类 号：R738.1[医药卫生—肿瘤]