Mina53蛋白在结肠腺瘤及结肠癌组织的表达及其临床意义  

Expression and significance of Mina53 protein in colon adenoma and colon carcinoma

作　　者：朱志坚[1] 李元平[1] 马志杰[1] 张志伟[1] 万灵燕[2] 王爱民[1] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]解放军252医院病理科,河北保定071000

出　　处：《中华实验外科杂志》2017年第2期294-296,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测Mina53蛋白在结肠腺瘤及结肠癌组织的表达，探讨其临床意义。 方法收集结肠腺瘤共90例，其中包括管状腺瘤30例，管状绒毛状腺瘤30例，绒毛状腺瘤30例；结肠癌组织60例和癌旁正常组织60例，应用免疫组织化学方法检测组织中Mina53蛋白的表达，同时利用聚合酶链反应（PCR）方法检测组织中Mina53 RNA的表达水平。 结果免疫组织化学结果显示Mina53在结肠正常黏膜、结肠不同类型腺瘤、结肠癌中的表达阳性率分别为0.0%、35.6%、68.3%（P＝0.000）；在管状腺瘤、混合性腺瘤、绒毛状腺瘤中的表达阳性率分别为26.7%、33.3%、50.0%（P＝0.001）；荧光定量PCR结果显示Mina53在正常结直肠黏膜、管状腺瘤、混合性腺瘤、绒毛状腺瘤及结肠癌组织中依次增高，其表达量分别为9.016 8±0.720 3、31.788 5±3.681 6、43.121 4±4.937 8、45.963 4±4.313 7、49.900 5±6.131 1（P＝0.016）。 结论结肠癌中Mina53蛋白表达显著高于各型腺瘤和正常黏膜组织，提示Mina53蛋白在结肠癌发生发展中具有重要作用。Objective To investigate the expression and clinical significance of MinaS3 protein in colon adenoma and colon carcinoma. Methods Ninety colon adenoma samples of different pathological types were collected, including 30 cases of tubular adenoma, 30 cases of tubulovillous adenoma, 30 cases of villous adenoma and 60 cases of colon carcinoma. There were 60 cases of normal tissue specimens. The protein and mRNA levels of MinaS3 were detected by immunohistochemistry and Real - time quantitative PCR respectively. Results The positive expression of MinaS3 in normal mucosa group, colon adenoma group and colon carcinoma group was 0. 0% , 36. 7% and 68.3% , respectively （P =0. 000）. The positive expression of MinaS3 in tubular adenoma group, tubulovillous adenoma group and villous adenoma group was 26. 7% , 33.3% and 50. 0%, respectively （P =0. 001 ）. Fluorescence quantitative PCR showed that the expression of Mina53 mRNA in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma was 9. 016 8 ± 0. 720 3,31. 788 5 ± 3.681 6,43. 121 4 ± 4.9378,45.9634±4.3137,49.9005±6.131 1 respectively （P=0. 016）. Conclusion The expression of Mina53 protein is higher in colon cancer than the colon adenoma and normal colon mucosa, suggesting that Mina53 may have a functional role in colon carcinogenesis and may be used as a marker for colon carcinoma.

关 键 词：结肠腺瘤 结肠癌 免疫组织化学 MINA53 

分 类 号：R735.35[医药卫生—肿瘤]