紧密连接蛋白闭锁蛋白在大肠癌及大肠腺瘤组织的表达  

Expression of tight junction proteins Occludin in colorectal carcinoma and colorectal adenoma

作　　者：马志杰[1] 朱志坚[1] 樊丽伟[1] 张东姣[1] 曹伟[1] 刘艺[2] 王爱民[1] 

机构地区：[1]解放军第252医院消化科,河北保定071000 [2]解放军第252医院病理科,河北保定071000

出　　处：《中华实验外科杂志》2017年第2期297-299,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测紧密连接蛋白闭锁蛋白（Occludin）在正常大肠黏膜、不同病理分型的大肠腺瘤和不同分期的大肠癌组织的表达，探讨其在大肠癌变过程中不同阶段表达的意义。 方法收集内镜下切除的不同病理分型的大肠腺瘤共80例，其中包括管状腺瘤30例，管状绒毛状腺瘤30例，绒毛状腺瘤20例；以及普通外科手术切除的大肠癌组织60例和癌旁正常组织40例，应用免疫组织化学方法检测组织中Occludin蛋白的表达。 结果Occludin在正常大肠黏膜组、大肠腺瘤组、大肠癌组中表达差异有统计学意义（P＝0.003），其阳性表达率分别为95.0%、88.7%、71.7%。并且在大肠癌组织中的表达与组织分化程度有关。在管状腺瘤、混合性腺瘤、绒毛状腺瘤中其阳性表达率分别为90.0%、86.7%、90.0%，差异无统计学意义（P＝0.901）。 结论Occludin在大肠癌组织中表达降低，其表达抑制肿瘤的发生、发展。Objective To detect the expression and implication of Oecludin protein in the normal mucosa and eoloreetal adenoma with different pathological types and different stages. Methods A total of 80 colorectal adenoma samples of different pathological type removed under endoscopy were collected, in- eluding 30 eases of tubular adenoma, 30 eases of tubular villous adenoma, and 20 cases of villous adeno- ma. Furthermore, the samples also included 60 cases of colorectal carcinoma specimens and 40 cases of paraeancerous normal tissue specimens that were cut off by general surgery. The immunohistochemistry was used to detect the expression of Oceludin protein. Results The positive rate of Oceludin expression in nor- mal mueosa group, eolorectal adenoma group and colorectal carcinoma group was 95.0%, 88.7% and 71.7%, respectively （P = 0. 003 ）. The expression of Occludin is associated with the degree of differentia- tion. The positive rate of Oecludin expression in tubular adenoma group, mixed pattern of adenoma group and villous adenoma group was 90.0%, 86. 7% and 90. 0% , respectively （ P = 0. 901 ）. Conclusion The expression of Oceludin in coloreetal carcinoma is reduced, and inhibited the occurrence and develop- ment of eoloreetal carcinoma.

关 键 词：大肠腺瘤 大肠癌 免疫组织化学 紧密连接蛋白 闭锁蛋白 

分 类 号：R735.34[医药卫生—肿瘤]