miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭  

miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1

作　　者：唐曦[1] 胡娅[2] 徐炎华[1] 汪春林[1] 邱萍[1] 王向辉[3] 

机构地区：[1]华中科技大学同济医学院附属荆州医院肿瘤科,湖北荆州433020 [2]长江大学医学院,湖北荆州433023 [3]华中科技大学同济医学院附属荆州医院胸外科,湖北荆州433020

出　　处：《西安交通大学学报（医学版）》2017年第2期226-230,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：湖北省教育厅项目(No.B2013280);湖北省卫生厅项目(No.2012Z-Y31)~~

摘　　要：目的探索微小RNA498(miR-498)是否通过下调FOXM1抑制肺癌细胞系(A549)细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况。结果与空质粒组比较,转染miR-498mimic的A549细胞中COL1A1、COL1A5、FOXM1的表达减少(P<0.05),A549细胞的迁移侵袭减少(P<0.01);荧光素酶实验结果显示,miR-498能够显著降低FOXM1-3′-UTR质粒的荧光素活性(P<0.05);与miR-498+空质粒组比较,转染过表达FOXM1质粒的A549细胞中COL1A1、COL1A5表达增加(P<0.05),细胞的迁移侵袭增加(P<0.01)。结论miR-498可以通过下调FOXM1而抑制A549的迁移和侵袭。Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by downregulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion abilities of A549 cells were decreased in the miR-498 group（both P〈0.01）.The luciferase activity of the FOXM1-3′-UTR plasmid was significantly suppressed by miR-498（P〈0.05）;over-expression of FOXM1 could reverse the effect of miR-498 on A549 cells.Conclusion miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1.

关 键 词：肺腺癌 微小RNA498(miR-498) COL1A1 COL1A5 FOXM1 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]