姜黄素对原发性肝癌模型大鼠组织VEGF及微血管密度影响研究  

Study on the Effects of VEGF and Microvascular Density by Curcumin on Primary Liver Cancer in Model Rats Tissue

作　　者：孟崇[1] 

机构地区：[1]中国医科大学附属盛京医院滑翔院区,辽宁沈阳110002

出　　处：《辽宁中医药大学学报》2017年第2期19-22,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省博士启动基金项目(20121134)

摘　　要：目的:观察姜黄素对原发性肝癌模型大鼠组织VEGF及微血管密度影响。方法:将45只Wistar大鼠分为正常对照组、模型对照组、姜黄素组各15只,正常对照组正常饲养,以间断小剂量二乙基亚硝胺溶液(DEN)诱发大鼠肝癌作为模型对照组,以在模型建立的同时接受姜黄素干预的大鼠作为姜黄素组。比较各组血清中低氧诱导因子-1α、血管内皮生长因子、肝功能指标以及微血管密度。结果:模型对照组以及姜黄素组血清中低氧诱导因子-1α、血管内皮生长因子、谷丙转氨酶以及谷草转氨酶均明显高于正常对照组(P<0.05);姜黄素组血清中上述指标明显低于模型对照组(P<0.05)。正常对照组肝组织血管内皮生长因子表达不明显,模型对照组肝组织血管内皮生长因子呈高表达,姜黄素组血管内皮生长因子表达降低(P<0.05)。模型对照组以及姜黄素组微血管密度明显高于正常对照组(P<0.05);姜黄素组微血管密度明显低于模型对照组(P<0.05)。结论:姜黄素可有效抑制原发性肝癌模型大鼠的血管生成,降低微血管密度,降低VEGF表达。Objective：To observe the effects of VEGF and microvascular density by curcumin on primary liver cancer model rats tissue. Methods ： A total of 45 Wistar rats were divided into normal control group, model control group, curcumin group with 15 rats in each group, normal control group with normal breeding, with interrupted small doses of two diethyl nitrosamine solution （ DEN ） induced ratsliver cancer as a model control group, at the same time accepted the curcumin intervene in model rats as curcumin group. Compared theserum hypoxia inducible factor-1 α , vascular endothelial growth factor, liver function index and microvaseular density of every group. Results ： The model control group and curcumin group of serum hypoxia inducible factor -1 α , vascular endothelial growth factor, alanine aminotransferase and glutamic oxalacetic transaminase were significant higher than the normal control group （ P〈0.05 ）; the above indexeslevels of curcumin group were obvious lower than that of model control group （ P〈0.05 ）. Normal control group of liver tissue vascular endothelial growth factor expression was not obvious, model control group of liver tissue vascular endothelial growth factor expressionwas high, curcumin group of vascular endothelial growth factor expression reduced （ P〈0.05 ）. Model control group and curcumin group of the microvascular density were obvious higher than that of normal control group （ P〈0.05 ）; Microvascular density of curcumin group was obvious lower than that of model control group （ P〈0.05 ）. Conclusion ： Curcumin can effectively inhibit primary liver cancer model rats angiogenesis, reduce microvascular density, reduce VEGF expression.

关 键 词：姜黄素 原发性肝癌 血管内皮生长因子 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤]