槐耳清膏和TOR逆转人肺腺癌细胞顺铂耐药性实验研究  

Reversal Effect of Polysaccharide of Trametes Robininophlia Murr(PS-T)and TOR in Human Lung Adenocarcinoma Cell with Resistance to Cisplatin

作　　者：程万宏[1] 王艳俊[1] 应朝辉[1] 隗玉川[1] 

机构地区：[1]大连大学附属新华医院,辽宁大连116022

出　　处：《辽宁中医药大学学报》2017年第2期23-25,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：大连市卫生局科研立项基金资助项目(WSJ/KJC-014-JL-82)

摘　　要：目的:研究槐耳清膏体外逆转人肺腺癌细胞株A2/c DDP耐药性的作用,并与TOR比较,探讨其可能机制。方法:MTT法确定槐耳清膏最适逆转浓度和处理前后A2/c DDP细胞对顺铂的敏感性,并与托瑞米芬(TOR)比较;RT-PCR测定CTR1和GSTπ基因表达变化。结果:槐耳清膏最适逆转浓度为0.6 mg/m L,此时A2和A2/c DDP细胞抑制率均<5%;槐耳清膏和TOR的半数抑制率(IC50)分别为(4.21±0.005)mg/m L和(39.06±0.0115)μmol/m L,逆转倍数(RI)分别为2.95±0.023和3.15±0.014,均能明显逆转A2/c DDP耐药性,P<0.05;而两者间比较无明显差异,P>0.05;槐耳清膏能明显增高A2/c DDP细胞CTR1表达并抑制GSTπ的表达。结论:槐耳清膏能体外逆转A2/c DDP对顺铂的耐药性,其逆转能力与TOR相近;上调CTR1和下调GSTπ表达可能是其相关机制。Objective ： To evaluate the reversal effects of PS-T and TOR in human lung adenocarcinoma cell line A2/cDDP, in vitro, and its possible mechanisms. Methods： MTT was used to detect the best dose of PS-T and the reversal ability of PS-T and TOR in A2/cDDP cells. RT-PCR was used to detect the expression of CTR1 and GST π. Results： While under the dose of 0.6 mg/mL, the inhibition rate caused by PS-T was less than 5%. Both PS-T and TOR could reverse the resistance of cisplatin in A2/cDDP cells significantly （P〈0.05）.The value of IC50 was （4.21 ± 0.005 ）mg/mL and （39.06± 0.0115 ） μmol/mL, RI was 2.95 μ 0.023 and 3.15 μ 0.014. In the ability of reversal effect, there was no significant difference between PS-T and TOR （ P〉0.05 ）. The expression of CTR1 was up-regulated and GST IT was downregulated. Conclusion： PS-T could reversed the resistance of cisplatin in human lung adinocareinoma cell line A2/cDDP and its ability was similar to TOR, in vitro. Up-regulated the expression of CTR1 and downregulated GST π may be its possible mechanisms.

关 键 词：顺铂耐药肺腺癌细胞株A2 槐耳清膏 托瑞米芬 铜特异性转运蛋白1 谷胱甘肽S-转移酶 

分 类 号：R734.2[医药卫生—肿瘤]