TIP30基因表达对人多发性骨髓瘤细胞生物学功能的影响  

The Effects of TIP30 Gene Expression on Biological Functions of Human Multiple Myeloma Cells

作　　者：吴必嘉[1] 龚峻梅[1] 陈娟娟[1] 黄斯勇[2] 高飞 

机构地区：[1]中国人民解放军第四二一医院肿瘤血液科,广东广州510318 [2]第四军医大学唐都医院血液科,陕西西安710038 [3]广州普康基因科技有限公司,广东广州510045

出　　处：《肿瘤药学》2017年第1期7-11,共5页Anti-Tumor Pharmacy

基　　金：国家自然科学基金资助项目(81300397)

摘　　要：目的研究TIP30基因表达对人多发性骨髓瘤U266细胞增殖、凋亡等生物学功能的影响,以期为多发性骨髓瘤临床防治及药物研制提供新的靶点。方法人多发性骨髓瘤U266细胞中TIP30基因过表达采用腺病毒转染法,使用实时定量PCR和Western blotting验证TIP30过表达的效果,分别采用MTT法、划痕试验检测细胞增殖、迁移情况,流式细胞术分析细胞凋亡,Western blotting分析p53、Bax、Bcl-2蛋白表达。结果 TIP30过表达腺病毒转染后,过表达组中U266细胞TIP30基因m RNA和蛋白表达水平均显著上升。与对照组比较,TIP30过表达后,细胞增殖、体外迁移能力均显著减弱(P<0.05);TIP30过表达后U266细胞凋亡率较对照组显著增大,TIP30过表达促进U266细胞凋亡与上调p53、Bax蛋白表达,下调Bcl-2蛋白表达水平相关。结论 TIP30基因过表达可抑制人多发性骨髓瘤U266细胞增殖活性与体外迁移能力,并促进多发性骨髓瘤细胞凋亡。Objective To study the effects of TIP30 gene overexpression on biological functions like cell proliferation and apoptosis of human multiple myeloma U266 cells, in order to provide a new target for clinical prevention and treatment, or drug research and develop- ment for multiple myeloma. Methods Adenovirus transfection was used to realize the TIP30 gene overexpression in human multiple my- eloma U266 cells. TIP30 gene overexpression was verified by real-time PCR and western blotting. The cell proliferation was analyzed by MTT method, and wound scratch assay were used to detect cell migration ability. The cell apoptosis rate was detected by flow cytometry. The apoptotic proteins expression levels of p53, Bax and Bcl-2 were detected by western blotting. Results The mRNA and protein expres- sion levels of gene TIP30 in human muhiple myeloma U266 cells significantly increased after overcxpression adenovirus transfcction. After the overexpression of TIP30, the cell proliferation and migratory ability of U266 cells in vitro decreased, as compared with the control group （P〈0.05）. In addition, the cell apoptosis rate significantly increased after TIP30 gene overexpression which is related to the up-regulation of p53 and Bax protein expression and the down-regulation of Bcl-2 expression. Conclusion The overexpression of gene TIP30 in human multiple myeloma U266 cells could inhibit the cell proliferation and migration ability in vitro, and enhance the cell apoptosis rate of U266 cells.

关 键 词：人多发性骨髓瘤细胞 TIP30基因 细胞增殖 凋亡 迁移 

分 类 号：R733.3[医药卫生—肿瘤]