靶向siRNA干扰hIAP-2联合姜黄素抑制肾癌GRC-1细胞增殖的实验研究  

Downregulation hIAP-2 with siRNA Enhance the Chemosensitivity of Renal Cancer GRC-1 Cell Line to Curcumin

作　　者：印重任 李远伟[2] 吴万瑞[2] 卢强[2] 李卓[2] 刘旭[1] 

机构地区：[1]南华大学,湖南衡阳421001 [2]湖南省人民医院泌尿外科,湖南长沙410001

出　　处：《肿瘤药学》2017年第1期33-37,共5页Anti-Tumor Pharmacy

摘　　要：目的应用hIAP-2特异性siRNA干扰肾癌GRC-1细胞hIAP-2基因的表达,探讨siRNA-hIAP-2联合姜黄素对肾癌GRC-1细胞增殖和凋亡的影响。方法体外化学合成针对hIAP-2的siRNA,通过阳离子脂质体将siRNA-hIAP-2转染肾癌GRC-1细胞,实时荧光定量PCR检测hIAP-2 m RNA表达水平,免疫印迹检测其蛋白表达水平,MTT法及流式细胞术分别测定siRNA-hIAP-2联合姜黄素处理后对肾癌GRC-1细胞增殖及凋亡的影响。结果靶向hIAP-2的siRNA可以有效抑制肾癌GRC-1细胞的hIAP-2的表达,siRNA-hIAP-2和姜黄素均可抑制GRC-1细胞增殖并诱导其一定程度的凋亡,当联合应用靶向hIAP-2的siRNA和姜黄素时,上述作用显著增强(P<0.05)。结论体外化学合成的siRNA-hIAP-2可以有效抑制肾癌GRC-1细胞的hIAP-2的表达,并能显著增强姜黄素诱导肾癌细胞GRC-1凋亡的敏感度,提示hIAP-2可作为姜黄素治疗肾癌的有效增敏靶点。Objective To investigate the therapeutic effect of targeting siRNA-hIAP-2 combined curcumin against GRC-1 cell line. Methods Renal cancer cell GRC-1 was cultured and transfected with siRNA-hIAP-2. Then real time PCR and Western blotting were performed to detect the changes of mRNA and protein of hIAP-2. Finally, MTT assay and flow cytometry were used to analyse the prolif- eration and apoptosis of targeting hIAP-2 combined curcnmin against GRC-1 cell line. Result SiRNA-hIAP-2 could down-regulate the expression of hIAP-2 mRNA and protein. Both cureumin and siRNA-hIAP-2 could induce apoptosis and inhibit proliferation in GRC-1 cell line. Moreover, a synergic effect against proliferation and survival of combined siRNA-hIAP-2 with cureumin in GRC-1 cell line was noted. Conclusion Downregulation of the expression of hlAP-2 could enhance the chemosensitivity of GRC-1 to curcumin, suggesting that hlAP-2 could be a potential therapeutic target to sensitize renal cell carcinoma to curcumin.

关 键 词：hIAP-2 肾癌 RNA干扰 姜黄素 GRC-1细胞 

分 类 号：R737.11[医药卫生—肿瘤]