Wnt/β-catenin信号通路在前列腺癌干细胞中的表达  

Expression and significance of signal pathway Wnt/β-catenin in prostate cancer stem cells

作　　者：柯莽[1] 洪涛[2] 俞洪元[1] 张显军[1] 黄汀[1] 

机构地区：[1]浙江温州医科大学附属台州医院泌尿外科,317000 [2]浙江温州医科大学附属台州医院泌尿外科特需病区,317000

出　　处：《中华内分泌外科杂志》2017年第1期74-77,85,共5页Chinese Journal of Endocrine Surgery

基　　金：台州市科技计划A类（15yw01）

摘　　要：目的观察Wnt/β-catenin信号通路在前列腺癌（prostate cancer，PCa）干细胞中的表达及意义。方法通过收集PCa和前列腺增生切除术患者组织标本和正常前列腺组织标本，应用肿瘤球悬浮分选法分选PCa干细胞，应用免疫组化、Western Blot和RT-PCR技术检测患者PCa干细胞中Wnt，β-catenin蛋白及mRNA的表达变化。结果免疫组化示Wnt和β-catenin蛋白在PCa组织中较前列腺增生组织和正常前列腺组织明显高表达；RT-PCR结果示PCa干细胞中Wnt mRNA（4.57±0.83）和β-catenin mRNA（3.93±0.78）水平较前列腺增生组织（1.32±0.35、1.48±0.44）和正常前列腺组织明显增高（1.00±0.12、1.00±0.11），差异有统计学意义（F=13.287、12.648，P均=0.000）；Western Blot结果示PCa干细胞中Wnt（0.87±0.10）和β-catenin（1.12±0.23）蛋白水平较前列腺增生组织（0.39±0.08、0.64±0.11）和正常前列腺组织（0.33±0.09、0.45±0.10）明显增高，差异有统计学意义（F=16.625、14.417，P均=0.000）。结论研究表明PCa的发生可能由于PCa干细胞中Wnt/β-catenin信号通路的激活引起异常增殖和分化导致,为PCa的治疗提供新靶点。ObjectiveTo investigate the expression and significance of signal pathway Wnt/β-catenin in prostate cancer stem cells.MethodsProstate cancer tissues, hyperplasia prostate tissues and normal prostate tissues were collected, then prostate cancer stem cells were selected from cell suspension in the culture system of serum-free medium by magnetic activated cell sorting system. Immunohistochemical SP test, RT-PCR and Western blot were applied to test the expression of Wnt and β-Catenin mRNA or protein in prostate cancer stem cells, hyperplasia prostate tissues and normal prostate tissues.ResultsThe protein expression of Wnt and β-Catenin was higher in prostate cancer tissues compared with that in hyperplasia prostate tissues and normal prostate tissues; mRNA expression of Wnt and β-Catenin was higher in prostate cancer stem cells （4.57±0.83, 3.93±0.78） than in hyperplasia prostate tissues （1.32±0.35, 1.48±0.44） and normal prostate tissues （1.00±0.12, 1.00±0.11） ,and the difference was statistically significant （F=13.287, 12.648, P=0.000） . Protein expression of Wnt and β-Catenin was higher in prostate cancer stem cells （0.87±0.10, 1.12±0.23） than in hyperplasia prostate tissues （0.39±0.08, 0.64±0.11） and normal prostate tissues （0.33±0.09, 0.45±0.10） , and the difference was statistically significant （F=16.625, 14.417, P=0.000） .ConclusionSignal pathway Wnt/β-catenin is stimulated abnormally in prostate cancer stem cells, causing the occurrence of prostate cancer, providing a new research direction for treatment of prostate cancer.

关 键 词：WNT Β-CATENIN 前列腺癌干细胞 

分 类 号：R737.25[医药卫生—肿瘤]