干细胞因子联合抗白细胞介素17A抗体对小鼠脑缺血预后的影响  

Effect of stem cell factor combined with anti-interleukin 17A antibody on prognosis of cerebral ischemia in mice

作　　者：卢龙[1] 贺伟峰[2] 王益[1] 文珊[1] 胡俊[1] 史树贵[1] 

机构地区：[1]第三军医大学西南医院神经内科,重庆400038 [2]第三军医大学西南医院全军烧伤研究所、创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2017年第5期455-459,共5页Journal of Third Military Medical University

摘　　要：目的探讨干细胞因子(stem cell factor,SCF)联合抗白细胞介素-17A抗体(anti-IL-17A)对小鼠脑缺血预后的影响。方法通过线栓法对C57BL/6小鼠建立大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型,60 min后取出线栓,恢复右侧大脑中动脉血流,建立脑缺血再灌注模型。小鼠脑缺血再灌注后分别给予腹腔注射生理盐水(normal saline,NS)、anti-IL-17A、SCF、anti-IL-17A+SCF。观察各组小鼠MCAO术后脑梗死体积、脑组织病理损伤和脑水肿程度、神经功能评分、神经细胞凋亡和炎症反应等变化。结果与NS组相比,其余各治疗组脑梗死体积、脑组织病理损伤和脑水肿均显著减轻(P<0.05),神经功能评分得到明显改善(P<0.05),神经细胞凋亡减少(P<0.05),且antiIL-17A+SCF组显著优于anti-IL-17A组和SCF组(P<0.05)。此外,anti-IL-17A还能明显减少脑组织IL-17A和IL-1β的表达(P<0.05)。结论 SCF联合anti-IL-17A能更好地改善脑缺血损伤和促进神经功能恢复。Objective To determine the effect of stem cell factor （SCF） combined with anti- interleukin 17A （anti-IL-17A） antibody on the prognosis of cerebral ischemia in mice. Methods Mouse model of middle cerebral artery occlusion （MCAO） were induced in C57BL/6 mice by inserting nylon monofilament through the right common carotid artery for 60 min followed by reperfusion. Then the mice were respectively given normal saline （NS）, anti-IL-17A, SCF, anti-IL-17A＋SCF through intraperitoneal injection after cerebral ischemia and reperfusion. The changes of cerebral infarct volumes, pathological injury severity and brain edema, neurological deficits, apoptosis and inflammatory response of neural cells after MCAO were observed in each group. Results Compared with the NS group, the cerebral infarct volume, pathological injury severity and brain edema were significantly alleviated in the other treatment groups （P 〈 O. 05 ）. The neurological deficits were significantly improved （ P 〈 0.05 ）, and the apoptosis of neurons was decreased in the treated groups （P 〈 0.05）. Furthermore, the effect of anti-IL-17A ＋ SCF treatment was better than that of anti-IL-17A and SCF alone treatment （P 〈 O. 05 ）. In addition, anti-IL-17A significantly reduced the expression levels of IL-17A and IL-1β in the brain tissues. Conclusion SCF combined with anti-IL-17A can better improve the cerebral ischemia injury and promote the recovery of neurological function.

关 键 词：脑缺血 炎症 白细胞介素-17 干细胞因子 

分 类 号：R-332[医药卫生] R743.31