茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响  

Effects of Epigallocatechin-3-gallate on Oxidative Stress and Inflammatory Factors in the Kidney Tissues of Rats with Paraquat Poisoning

作　　者：沈海涛[1] 吴娜[2] 赵宏宇[1] 胡晓[1] 郭峰[1] 赵敏[1] 

机构地区：[1]中国医科大学附属盛京医院急诊科,沈阳110004 [2]中国医科大学附属盛京医院内分泌科,沈阳110004

出　　处：《中国医科大学学报》2017年第3期210-213,218,共5页Journal of China Medical University

基　　金：卫生部国家临床重点专科建设项目(2012-649)

摘　　要：目的探讨茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响及可能机制。方法将大鼠随机分为对照组(A组)、百草枯染毒组(B组)、百草枯染毒+小剂量茶多酚组(C组)、百草枯染毒+大剂量茶多酚组(D组),每组10只。HE染色观察肾脏组织,生化检测仪检测肌酐水平,ELISA法检测血清中8-异前列腺素、白细胞介素6(IL-6)及肿瘤坏死因子α(TNF-α)水平,Western blotting检测3-硝基酪氨酸(3-NT)蛋白水平,比色法检测肾脏组织氧化应激水平,实时PCR检测肾脏组织TNF-αmRNA及IL-6 mRNA水平。结果 B、C、D组大鼠在百草枯染毒后均出现肾脏受损,但C、D组在茶多酚干预下肾脏损伤较B组有所改善,且大剂量茶多酚干预下的D组肾脏损伤改善更为明显。在茶多酚干预下的C、D组血清中肌酐、8-异前列腺素、IL-6及TNF-α水平以及肾脏组织的丙二醛、3-NT、TNF-αmRNA及IL-6 mRNA水平较B组均降低,大剂量茶多酚干预下的D组降低更为显著。结论茶多酚干预对百草枯中毒大鼠肾脏有保护作用,其作用机制可能与减轻肾脏中氧化应激及炎症反应有关。Objective To investigate the effect of Epigallocatechin-3-gallate（EGCG）on oxidative stress and inflammatory factors in the kidney tissues of rats with paraquat poisoning,and explore the possible mechanism. Methods Rats were randomly divided into control group（group A）,paraquat exposure group（group B）,paraquat exposure＋low dose of EGCG group（group C）,and paraquat exposure＋high dose of EGCG group（group D）（n = 10 in each group）. Kidney tissues were harvested and observed by HE staining. The level of creatinine was tested by biochemical detector. Serum oxidative stress and inflammatory factor level were detected by ELISA method. Oxidative stress in kidney tissue was determined by Western blotting and colorimetry. Inflammatory factor level in renal tissue was tested by real-time PCR. Results Kidney damage was observed in rats of groups B,C,and D. Rats in groups C and D showed less renal injury than group B,and high dose of EGCG（group D）enhanced kidney damage compared to the low dose（group C）. Compared with group A,rats in the groups C and D showed lower level of 8-isoprostane,creatinine,interleukin-6（IL-6）,and tumor necrosis factor α（TNF-α）in serum and malondialdehyde（MDA）,3-nitrotyrosine（3-NT）,and TNF-α and IL-6mRNA in renal tissue in rats with paraquat poisoning,and group D showed the lowest mRNA level of inflammatory factor and oxidative stress. Conclusion EGCG has protective effects on the kidney of rats with paraquat poisoning,and its mechanism may be related to the reduction of oxidative stress and inflammatory reaction in the kidney.

关 键 词：茶多酚 百草枯 氧化应激 炎症反应 肾脏 

分 类 号：R595.4[医药卫生—内科学]