高尔基体磷蛋白3在肝细胞癌中的表达特点及其临床病理学意义  

Expression of Golgi Phosphoprotein 3 in Human Hepatocellular Carcinoma and Its Clinicopathological Significance

作　　者：张城硕[1] 陈保民[1] 焦奥[1] 孙宁[1] 张佳林[1] 

机构地区：[1]中国医科大学附属第一医院肝胆外科暨器官移植科,沈阳110001

出　　处：《中国医科大学学报》2017年第3期214-218,共5页Journal of China Medical University

基　　金：辽宁省自然科学基金(201602874)

摘　　要：目的探讨高尔基体磷蛋白3(GOLPH3)在肝细胞癌中的表达特点及其临床病理学意义。方法采用免疫组织化学方法检测132例肝细胞癌与配对的癌旁组织中GOLPH3蛋白的表达情况,分析肝细胞癌组织中GOLPH3表达水平与患者组织病理学特征的关系。利用激光共聚焦显微镜检测GOLPH3蛋白在肝细胞癌细胞的亚细胞定位。结果 GOLPH3在肝细胞癌组织中的阳性表达率为70.0%(92/132),在癌旁组织中为42.4%(56/132),GOLPH3在肝细胞癌组织中的表达水平明显高于其癌旁组织(P<0.001)。肝细胞癌组织中,GOLPH3高表达组门静脉癌栓的发生率为21.2%(14/66),而GOLPH3低表达组为6.1%(4/66),GOLPH3高表达组门静脉癌栓的发生率明显高于GOLPH3低表达组(P<0.05)。激光共聚焦免疫荧光结果显示GOLPH3主要存在于肝细胞癌细胞的细胞质中,且在细胞核内存在散在性分布。结论 GOLPH3可作为一种癌基因在肝细胞癌的发生发展中发挥重要作用。Objective To investigate the expression characteristics of Golgi phosphoprotein 3（GOLPH3）in human hepatocellular carcinoma（HCC）and explore its clinicopathological significance. Methods The expressions of GOLPH3 protein was detected in 132 cases of paired paraffin embedded HCC specimens and pericarcinoma tissues using immunohistochemical staining,and the relation of the expression of GOLPH3 to clinicopathologic features was analyzed. Meanwhile,the expression and distribution of GOLPH3 in HCC cells was observed by laser confocal microscopy. Results The positive expression rates of GOLPH3 in HCC and pericarcinoma tissues were 70.0%（92/132）and 42.4%（56/132）（P 0.001）,respectively. The incidence of portal vein tumor thrombus in high and low GOLPH3 expression groups of HCC were 21.2%（14/66）and 6.1%（4/66）（P 0.05）,respectively. The expression rate of GOLPH3 in HCC was significantly higher than that in pericarcinoma tissues,and the expression of GOLPH3 in HCC was positively related to portal vein tumor thrombus. In addition,GOLPH3 was mainly expressed in cytoplasm of HCC cells,and there was also scattered distribution in the nucleus. Conclusion GOLPH3 acts as an oncogene and may play vital roles in the carcinogenesis and development of HCC.

关 键 词：肝细胞癌 高尔基体鳞蛋白3 免疫组化 免疫荧光 

分 类 号：R735.7[医药卫生—肿瘤]