五谷虫提取物对H22肝癌细胞荷瘤小鼠的抗肿瘤机制研究  

Exploration of the anti-tumor effects and mechanisms of Chrysomya megacephala( Fab.) extracts in H22 hepatoma-bearing mice

作　　者：张文娟[1] 蒋伟哲[1] 蓝献丽 植国繁 蓝彬[1] 焦爱军[1] 巫玲玲[1] 

机构地区：[1]广西医科大学药学院,南宁市530021

出　　处：《广西医学》2017年第2期215-219,共5页Guangxi Medical Journal

基　　金：广西南宁市科学研究与技术开发计划(20163015);广西壮族自治区教育厅高校科技创新平台建设计划(桂教科研〔2013〕8号-3)

摘　　要：目的探讨五谷虫提取物对H22肝癌细胞荷瘤小鼠的抑瘤作用及其对p38MAPK信号通路的影响。方法构建H22荷瘤小鼠模型,随机分为正常组、模型组、阳性对照组、五谷虫提取物低、中、高剂量(0.75 g/kg、1.5 g/kg、3 g/kg)组,每组8只,造模24 h后,模型组小鼠给予0.5%的羧甲基纤维素钠灌胃;阳性药物组给予环磷酰胺腹腔注射;五谷虫提取物低、中、高剂量剂量组分别给予0.75 g/kg、1.5 g/kg、3 g/kg五谷虫溶液灌胃,连续给药10 d。观察各组小鼠肿瘤生长情况,计算肿瘤抑制率;检测小鼠血清ALT、AST、尿酸(UA)和肌酐(Cr)含量;免疫印迹法检测肿瘤组织中p38丝裂素活化蛋白激酶(p38MAPK)、p-p38MAPK蛋白表达水平;酶联免疫吸附法检测瘤组织中白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子(TNF-α)、血管内皮生长因子(VEGF)水平。结果阳性对照组、五谷虫提取物低、中剂量组均可抑制H22荷瘤小鼠肿瘤的生长(P<0.05)。五谷虫提取物各剂量组对小鼠肝肾功能指标无影响(P>0.05);各剂量组的瘤组织p-p38MAPK蛋白表达水平均高于模型组(P<0.05);低、中剂量组IL-1β、IL-6、TNF-α水平高于模型组(P<0.05),而VEGF水平低于模型组(P<0.05)。结论低、中剂量五谷虫提取物可抑制H22荷瘤小鼠体内的肿瘤生长,其抗肿瘤机制可能与调节肿瘤相关细胞因子及激活p38MAPK信号通路有关。Objective To investigate the anti-tumor effects of Chrysomya megacephala（ Fab.） Extracts in H22 tumor-bearing mice and its influence on p38 MAPK signal pathway. Methods H22 hepatoma-bearing mice models were established,and randomly divided into five groups： normal group,model group,positive control group,or Chrysomya megacephala（ Fab.） extracts low,middle,high dosage groups（ 0.75 g/kg,1.5 g/kg,3 g/kg）,with 8 in each group. Twenty-four hours after molding,model group was given sodium carboxymethyl cellulose,positive control group was given cyclophosphamide,while positive control groups were given corresponding dosage of Chrysomya megacephala（ Fab.） solution by continuous gavage for 10 days. The tumor growth situation of mice was observed and the tumor inhibition rate was calculated.ALT,AST,uric acid（ UA）,creatinine（ Cr） index of mice were determined. The protein expressions of p38 mitogen-activated protein kinase（ p38MAPK） and phosphorylated-p38MAPK（ p-p38MAPK） in tumor tissues were detected by Western blotting. The levels of interleukin（ IL）-1β,IL-6,tumor necrosis factor（ TNF）-α,and vascular endothelial growth factor（ VEGF） in tumors were detected by enzyme-linked immunosorbent assay. Results H22 hepatoma-bearing mice models were established,and randomly divided into five groups： normal group,model group,positive control group,or Chrysomya megacephala（ Fab.） extracts low,middle,high dosage groups（ 0. 75 g / kg,1. 5 g / kg,3 g / kg）,with 8 in each group.Twenty-four hours after molding,model group was given sodium carboxymethyl cellulose,positive control group was given cyclophosphamide,while positive control groups were given corresponding dosage of Chrysomya megacephala（ Fab.） solution by continuous gavage for 10 days. The tumor growth situation of mice was observed and the tumor inhibition rate was calculated. ALT,AST,uric acid（ UA）,creatinine（ Cr） index of mice were determined. The protein expressions of p38 mitogen-activated protein kinase�

关 键 词：肝肿瘤 五谷虫 P38丝裂素活化蛋白激酶 

分 类 号：R735.7[医药卫生—肿瘤]