Significant variations in alternative splicing patterns and expression profiles between human-mouse orthologs in early embryos  

作　　者：Geng Chen Jiwei Chen Jianmin Yang Long Chen Xiongfei Qu Caiping Shi Baitang Ning Leming Shi Weida Tong Yongxiang Zhao Meixia Zhang Tieliu Shi 

机构地区：[1]The Center for Bioinformatics and Computational Biology, Shanghai Key Laboratory of Regulatory Biology, the Institute of Biomedical Sciences and School of Life Sciences, East China Normal University [2]Center for Pharmacogenomics, School of Pharmacy, Fudan University [3]National Center for Toxicological Research, US Food and Drug Administration [4]Biological Targeting Diagnosis and Therapy Research Center, Guangxi Medical University [5]Department of Ophthalmology, West China Hospital, Sichuan University

出　　处：《Science China(Life Sciences)》2017年第2期178-188,共11页中国科学（生命科学英文版）

基　　金：supported by the China Human Proteomics Project (2014DFB30010);the National High Technology Research and Development Program of China (2015AA020104);the National Natural Science Foundation of China (31071162);the Graduate School of East China Normal University

摘　　要：Human and mouse orthologs are expected to have similar biological functions; however, many discrepancies have also been reported. We systematically compared human and mouse orthologs in terms of alternative splicing patterns and expression profiles. Human-mouse orthologs are divergent in alternative splicing, as human orthologs could generally encode more isoforms than their mouse orthologs. In early embryos, exon skipping is far more common with human orthologs, whereas constitutive exons are more prevalent with mouse orthologs. This may correlate with divergence in expression of splicing regulators. Orthologous expression similarities are different in distinct embryonic stages, with the highest in morula. Expression differences for orthologous transcription factor genes could play an important role in orthologous expression discordance. We further detected largely orthologous divergence in differential expression between distinct embryonic stages. Collectively, our study uncovers significant orthologous divergence from multiple aspects, which may result in functional differences and dynamics between human-mouse orthologs during embryonic development.Human and mouse orthologs are expected to have similar biological functions; however, many discrepancies have also been reported. We systematically compared human and mouse orthologs in terms of alternative splicing patterns and expression profiles. Human-mouse orthologs are divergent in alternative splicing, as human orthologs could generally encode more isoforms than their mouse orthologs. In early embryos, exon skipping is far more common with human orthologs, whereas constitutive exons are more prevalent with mouse orthologs. This may correlate with divergence in expression of splicing reg- ulators. Orthologous expression similarities are different in distinct embryonic stages, with the highest in morula. Expression differences for orthologous transcription factor genes could play an important role in orthologous expression discordance. We further detected largely orthologous divergence in differential expression between distinct embryonic stages. Collectively, our study uncovers significant orthologous divergence from multiple aspects, which may result in functional differences and dy- namics between human-mouse orthologs during embryonic development.

关 键 词：ORTHOLOG alternative splicing RNA-SEQ early embryo gene expression 

分 类 号：Q78[生物学—分子生物学] S823.3[农业科学—畜牧学]