SNS-032对紫杉醇耐药乳腺癌细胞生长的抑制作用研究  

Anti-proliferative effect of SNS-032 on taxol-resistant breast cancer cells

作　　者：谢闺娥[1] 杜晶春[1] 董桂兰[2] 徐霞[1] 

机构地区：[1]广州医科大学金域检验学院,广东广州510182 [2]广州大学门诊部,广东广州510006

出　　处：《海南医学》2017年第3期345-347,共3页Hainan Medical Journal

基　　金：国家自然科学基金(编号:81101682);广东省公益研究与能力建设专项资金(编号:2014A020212015)

摘　　要：目的研究细胞周期蛋白依赖性激酶抑制剂SNS-032对人紫杉醇耐药MCF-7/TAX乳腺癌细胞生长的作用。方法采用MTT试验检测MCF-7/TAX细胞对紫杉醇的耐药情况以及SNS-032对紫杉醇耐药MCF-7/TAX细胞生长的影响;采用Annexin V-FITC/PI双染试验检测SNS-032对MCF-7/TAX耐药细胞凋亡的诱导作用。结果 MCF-7/TAX细胞对紫杉醇耐药指数达19.39;而SNS-032能显著抑制MCF-7/TAX耐药细胞的增殖,且具有明显的浓度依赖性,对其作用48 h的IC50为164.5 nmol/L,MCF-7/TAX对SNS-032的耐药指数仅为0.89。SNS-032作用后,Annexin V染色阳性的MCF-7/TAX细胞比例较对照明显升高,差异具有统计学意义(P<0.05)。结论 SNS-032能激活MCF-7/TAX耐药细胞凋亡的发生,从而对紫杉醇耐药MCF-7/TAX细胞的生长发挥高效抑制作用。Objective To investigate the effect of the cyclin-dependent kinase inhibitor SNS-032 on the growth of taxol-resistant MCF-7/TAX breast cancer cells.Methods The resistance situation of MCF-7/TAX cells to paclitaxel and the effect of SNS-032 on the growth of taxol-resistant MCF-7/TAX cells were evaluated by MTT（3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide） assay.The effect of SNS-032 on apoptosis in MCF-7/TAX cells was analyzed by Annexin V-FITC/PI staining.Results The resistance index of MCF-7/TAX cells to paclitaxel was 19.39.After treatment with SNS-032 for 48 h,MCF-7/TAX cells displayed markedly inhibited growth in a dose-dependent manner,and calculated IC50 was 164.5 nmol/L.SNS-032 decreased the resistance index of MCF-7/TAX from19.39 to 0.89.The numbers of apoptotic MCF-7/TAX cells,as revealed by Annexin V binding,significantly increased upon SNS-032 treatment（P〈0.05）.Conclusion The results demonstrate that SNS-032 can induce apoptosis in MCF-7/TAX cells and consequently display potent cytocidal effect on taxol-resistant MCF-7/TAX breast cancer cells.

关 键 词：紫杉醇耐药 SNS-032 MCF-7/TAX 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]