基于共聚焦拉曼光谱技术的油菜菌核病早期判别分析  

Discriminate the Rape Sclerotinia at Early Stage Based on Confocal Raman Spectroscopy

作　　者：赵艳茹[1] 李晓丽[1] 余克强[2] 程帆[3] 刘吉强[4] 何勇[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [2]西北农林科技大学机械与电子工程学院,陕西杨凌712100 [3]浙江大学农业与生物技术学院,生物技术研究所,浙江杭州310058 [4]国家海洋局第二海洋研究所,浙江杭州310012

出　　处：《光谱学与光谱分析》2017年第2期467-471,共5页Spectroscopy and Spectral Analysis

基　　金：高等学校博士学科点专项科研基金项目(20130101110104);国家自然科学基金项目(31471417,31402318)资助

摘　　要：油菜菌核病是一种真菌性病害,可造成油菜产量严重减少,而快速准确地进行病原物的早期侵染对于植物病害防治意义重大。采用共聚焦拉曼光谱(500~2 000cm^(-1)波数范围内)技术结合化学计量学方法对油菜菌核病早期侵染进行判别分析。采用共聚焦拉曼光谱仪采集健康和接种12h核盘菌的油菜叶片表面拉曼光谱,应用小波变换(wavelet transform,WT)进行拉曼光谱预处理以去除荧光背景。并利用基于全谱范围的偏最小二乘判别分析(partial least squares-discriminant analysis,PLS-DA)的回归系数(regression coefficient,RC)进行特征峰的识别,选出983,1 001,1 205,1 521,1 527,1 658,1 670和1 758cm^(-1)共八个特征峰用于建立PLS-DA模型进行油菜菌核病的早期侵染判别,其识别准确率为100%。结果表明:拉曼光谱技术结合化学计量学方法能够实现油菜叶片中菌核病早期侵染的检测,这为后续探究核盘菌与油菜叶片互作过程以及为进一步的病害早期监测和预防提供理论参考。Oilseed rape（Brassica napus L.）is a principal source of edible oil for human consumption and it feeds livestock as a by product with high energy and protein.However,oilseed plants often suffer from the invasion of various diseases,which could affect the yield and quality of the rapeseeds.Rape sclerotinia rot caused by the fungus sclerotinia sclerotiorum（Lib.）de Bary may severely affect the growth of oilseed rape.Therefore,searching non-invasive detection methods of detection plant disease at early stage is crucial for monitoring growing conditions of crops.Confocal Raman spectroscopy in the region of 500-2 000cm^-1coupled with chemometrics methods were employed to discriminate the rape sclerotinia disease at early stage on the oilseed rape leaves.A total of 60samples（30healthy plant leaves and 30 infected leaves）were used to acquire the Raman spectra and wavelet transform was applied to remove the fluorescence background.Regression coefficients of the partial least squares-discriminant analysis（PLS-DA）were used to select the 8characteristic peaks based on the whole Raman spectra.983,1 001,1 205,1 521,1 527,1 658,1 670 and 1 758cm^-1 were employed to establish PLS-DA discriminate models and recognition accuracy was100%.The results showed Raman spectra combined with chemometrics method is promising for detecting rape sclerotinia infection in the oilseed rape leaves at early stage.This study provided a theoretical reference for researching the interaction between the fungus and plants and early detecting of disease infection.

关 键 词：油菜叶片 菌核病 拉曼光谱 化学计量学方法 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]