光谱法研究葫芦[6]脲与土霉素的分子识别作用（英文）  

Study on Molecular Recognition of Cucurbit[6]uril with Oxytetracycline Molecules by Spectroscopic Methods

作　　者：严昆[1,2] 王萍[1] 刘云[2] 董元华[2] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070 [2]中国科学院土壤环境与污染修复重点实验室,南京土壤研究所,江苏南京210008

出　　处：《光谱学与光谱分析》2017年第2期641-645,共5页Spectroscopy and Spectral Analysis

基　　金：The National Natural Science Foundation of China(21477137)

摘　　要：采用荧光光谱和紫外光谱研究葫芦[6]脲(CB[6])与土霉素(OTC)的分子识别作用。实验结果表明,随着主体分子的加入,OTC荧光强度和紫外吸收强度均增强,说明主客体之间发生了相互作用;进一步通过荧光光谱、紫外光谱和直线拟合法(Benesi-Hildebrand)确定了CB[6]与OTC形成1∶1型的包结物;在酸性环境下CB[6]与OTC能形成1∶1型的稳定的包结物,在碱性环境下不能形成稳定的包结物;同时计算了主客体在298,308和328K下包结的平衡常数Ks和CB[6]与OTC的分子识别过程的熵变、焓变和吉布斯自由能变。不同温度下的吉布斯自由能变ΔG均小于0,而熵变和焓变为正值,其包结过程是疏水作用力驱动且自发的吸热的过程。The molecular recognition of Cucurbit[6]uril（CB[6]）with Oxytetracycline（OTC）was studied by fluorescence spectra and UV spectra.The results showed that the fluorescence intensity and the UV absorbance of OTC were enhanced as host molecules were added,which showed that CB[6]and OTC were interacted.The inclusion of OTC was detected by fluorescence spectra and UV spectra with an apparent 1∶1interaction,which was also observed through the Benesi-Hildebrand method,OTC can form 1∶1complex with CB[6]under acid condition and it can not form complex with CB[6]under basic condition.The thermodynamic parameters（stability constants Ks,Gibbs free energy changeΔG,enthalpy change,and entropy change）for the complexation process were determined.The thermodynamic studies indicated that the inclusion reactions between CB[6]and OTC were the hydrophobic interaction and endothermic process.

关 键 词：脲 土霉素 分子识别 荧光光谱法 紫外光谱法 

分 类 号：R914[医药卫生—药物化学]