斑贞1号联合TMP、5-FU对人胃癌细胞CDX2基因表达影响  

Ban Zhen 1、TMP and 5-FU Effect on Human Gastric Cancer Cells CDX2 Gene Expression

作　　者：张爱民[1] 郭元虎[1] 郝旺胜[1] 马振华[1] 

机构地区：[1]包头医学院第一附属医院消化科,内蒙古包头014010

出　　处：《中外医疗》2017年第1期32-34,37,共4页China & Foreign Medical Treatment

摘　　要：目的探讨斑贞1号联合TMP、5-FU逆转人胃癌细胞系SGC-7901/ADR与CDX2相关性。方法从2015年6—12月在包头医学院第一附属医院中心实验室进行SGC7-901/ADR细胞培养,设1空白对照组、25-FU组、3斑贞1号组、45-FU+斑贞1号组、55-FU+斑贞1号+TMP组观察细胞毒性,选取各个药物组作用浓度分别提取总m RNA,采用半定量逆转录聚合酶链反应(RT-PCR)监测人胃癌细胞SGC-7901/ADR细胞在不同药物组的尾型同源基因2(caudal-re lated hom e-odom aintranscription factor 2,Cd X2)m RNA的表达情况。结果对照组CDX2 m RNA表达量为(2.01±0.26),5-FU组CDX2m RNA表达量为(1.94±0.24),5-FU组与阴性对照组相比差异无统计学意义(P>0.05),而5-FU联合斑贞1号及TMP处理组CDX2 m RNA表达量为(0.44±0.07),与其他组明显降低比较,差异有统计学意义(P<0.05)。结论斑贞1号联合TMP及5-FU逆转人胃癌SGC-7901/ADR细胞耐药性可能与CDX2基因相关。Objective To explore of the correlativity between Ban Zhen 1,Tetra methylpyrazine（TMP） and 5-Fu reversing human gastric cancer cell line SGC-7901/ADR with CDX2 expressions. Methods From June 2015 to December of SGC7-901/ADR cells in the First Affiliated Hospital of Baotou Medical College central laboratory training set the blank control group, 5-FU group, 3 banzhen 1 group, the 5-FU＋ group of banzhen 1, 5 5-FU＋ banzhen 1 ＋ TMP group to observe the cytotoxicity, choosing each drug group were extracted the total m RNA with concentration, using semi quantitative reverse transcriptase polymerase chain reaction（RT-PCR） monitoring tail type homologous gene of human gastric carcinoma SGC-7901/ADR cells in different drug group 2（caudal- re lated Hom e- Odom aintranscription factor 2, Cdx2） the expression of m RNA. Results CDX2 m RNA expression levels in the control group was（2.01±0.26）,and was（1.94±0.24） in 5-FU group,5-FU group compared with the control group was not statistically significant（P〈0.05）, and in Ban Zhen1 ＋ 5- FU ＋ TMP group CDX2 m RNA expression levels was（0.44±0.07）, reduced the other group, with statistical significance（P〈0.05）. Conclusion It is possible correlated to CDX2 for Ban Zhen 1,TMP and 5- FU reversed multidrug resistance in human gastric cancer cells.

关 键 词：胃癌 斑贞1号 TMP 5-FU 多药耐药 CDX2基因 

分 类 号：R735.2[医药卫生—肿瘤]