用固绿FCF作探针快速测定萝卜红色素中的Pb  

Fast determination of lead in radish red pigment with FCF as a probe

作　　者：江虹[1] 庞向东[1] 谭建红[1] 尤晓露[1] 陈娥[1] 

机构地区：[1]长江师范学院化学化工学院,重庆市无机特种功能材料重点实验室,重庆408100

出　　处：《食品与发酵工业》2017年第2期211-214,共4页Food and Fermentation Industries

基　　金：重庆市教委科技基金资助项目(KJ1401226);长江师范学院科技基金资助项目(2015CXX079)

摘　　要：建立了快速测定萝卜红色素中Pb的瑞利散射新方法。固绿FCF与阳离子表面活性剂十六烷基三甲基溴化铵在酸性Tris-盐酸缓冲介质中,与Pb(Ⅱ)结合生成的三元复合物能使瑞利散射(RLS)显著增强并产生新的RLS光谱,在最大RLS峰342 nm波长处,Pb(Ⅱ)的质量浓度在0.005~0.40 mg/L内与体系的瑞利散射增强程度△IRLS呈线性关系,定量限为0.044 mg/kg。方法用于萝卜红色素中Pb的测定,回收率为98.5%~102%,相对标准偏差RSD(n=5)为1.8%~2.3%。A new and fast Rayleigh light scattering method for quantified detection of lead in radish red pigment was developed. In an acidic Tris-Hydrochloric acid buffer medium, lead（Ⅱ） can be bound with fast green FCF and cationic surfactant cetyl trimethyl ammonium bromide to form a ternary complex and distinctively enhanced Rayleigh scattering （RLS） of the system and form a new RLS spectrum. The maximum Rayleigh scattering peak was located at 342 nm. In this wavelength, The RLS intensity （ △IRLS） was directly proportion to the mass concentration of lead （Ⅱ） in the range of 0. 005 -0.40 mg/L with the quantitative limit of 0. 044 mg/kg. The method applied in trace lead detection in radish red pigment with satisfied results as the recovery rate of 98.5% -102% and RSD（n = 5） of 1.8% -2.3%.

关 键 词：固绿FCF PB 萝卜红色素 十六烷基三甲基溴化铵 瑞利散射 

分 类 号：TS264.4[轻工技术与工程—发酵工程]