正交试验法优选葛根复方中挥发油的β-环糊精包合工艺  

Optimization of β-Cyclodextrin Inclusion Technology of Volatile Oil from Pueraria Compound by Orthogonal Design

作　　者：周晶[1] 李玲[1] 崔冬雪[1] 王冰[1] 张彤[1] 袁秀荣[1] 刘素嬛[2] 

机构地区：[1]上海中医药大学,上海201203 [2]厦门大学附属第一医院,福建厦门361003

出　　处：《时珍国医国药》2017年第1期105-108,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81270901);上海市教委基金(No.15cxy21)

摘　　要：目的优选葛根复方挥发油的包合工艺,提高制剂的稳定性。方法采用饱和水溶液法制备包合物,以挥发油中藁本内酯、桂皮醛、甲基丁香酚包合率及包合物得率为综合评价指标,采用正交试验法考察挥发油与β-环糊精的比例、包合时间、包合温度对包合工艺的影响,优化得到葛根复方中挥发油的最优包合工艺。结果葛根复方中挥发油的最佳包合工艺条件为挥发油与β-环糊精比例为1∶8,包合温度为30℃,包合时间为1h,验证试验中,藁本内酯、桂皮醛及甲基丁香酚的包合率为别为86.58%±0.009%,73.34%±0.001%,68.89%±0.020%,包合物得率为67.98%±0.047%。结论所确定的包合工艺挥发油的包合率高、工艺稳定可行。Objective To obtain the optimized technology of volatile oil from Pueraria Compound with β-Cyclodextrin（ β-CD）. Methods The inclusion compound was prepared by saturated peroxide solution method. In order to optimize inclusion technology,orthogonal design was performed,with the content of multi-target ingredient,such as ligustilide,cinnamic aldehyde,methyleugenol and the yield of inclusion as comprehensive evaluation indexes. Three factor was performed,the ratio of volatile oil（ m L） to β-CD（ g）,the inclusion temperature and the inclusion time. Results The optimum conditions for inclusion process were as follow： the ratio of volatile oil（ m L） to β-CD（ g） was 1： 8,the inclusion temperature was 30℃ and the inclusion time was 1h. With the optimum inclusion condition,the content of ligustilide,cinnamic aldehyde and methyleugenol and the yield of inclusion were 86. 58% ± 0. 009%,73. 34% ± 0. 001%,68. 89% ± 0. 020%,and 67. 98% ± 0. 047%,respectively.Conclusion The optimum inclusion condition was stable and reproducible with a high inclusion yield.

关 键 词：葛根复方 挥发油 Β-环糊精 正交试验 

分 类 号：R283[医药卫生—中药学]