Prevalence of ocular demodicosis among patients at Tertiary Care Center,Bangkok,Thailand  

作　　者：Ngamjit Kasetsuwan Kitchaporn Kositphipat Mathu Busayarat Pawanrat Threekhan Kanok Preativatanyou Atchara Phumee Padet Siriyasatien 

机构地区：[1]Department of Ophthalmology,Faculty of Medicine,Chu-lalongkorn University,Bangkok 10330,Thailand [2]National Institute of Health,Department of Medical Sciences,Ministry of Public Health,Nonthaburi 11000,Thailand [3]Department of Parasitology,Faculty of Medicine,Chulalong-korn University,Bangkok 10330,Thailand [4]Excellence Center for Emerging Infectious Disease,King Chulalongkorn Memorial Hospital,Thai Red Cross Society,Bangkok 10330,Thailand

出　　处：《International Journal of Ophthalmology(English edition)》2017年第1期122-127,共6页国际眼科杂志（英文版）

基　　金：Supported by National Science and Technology Development Agency(Thailand)for a Research Chair Grant

摘　　要：AIM： To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok.METHODS： One hundred individuals were enrolled in the study and were divided into five age groups. The meibomian gland dysfunction （MGD） score and qualities of cylindrical dandruff （CD） were also determined. Demodex mite infestations of eyelash samples were screened by both microscopic examination and semi-nested polymerase chain reaction （PCR）. RESULTS： The prevalence of ocular demodicosis as determined by microscopic examination was 42% [Demodex folliculorum （D. folliculorum） 41% and Demodex brevis （D. brevis） 1%]. Among patients who had ocular Demodex infestation, 69% have CD and had an average MGD score of 4; in patients without demodicosis, 15.5% had CD and had an average MGD score of 4.12. Prevalence of ocular demodicosis as determined by semi-nested PCR was 79% （D. folliculorum 78% and D. brevis 1%）.CONCLUSION： This is the first report on the prevalence of ocular demodicosis in Thailand. Patients with CD also had Demodex mites present. Semi-nested PCR is better than microscopy for Demodex infestation detection. An extensive survey with more representative samples is required to determine the prevalence in the country.AIM： To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok.METHODS： One hundred individuals were enrolled in the study and were divided into five age groups. The meibomian gland dysfunction （MGD） score and qualities of cylindrical dandruff （CD） were also determined. Demodex mite infestations of eyelash samples were screened by both microscopic examination and semi-nested polymerase chain reaction （PCR）. RESULTS： The prevalence of ocular demodicosis as determined by microscopic examination was 42% [Demodex folliculorum （D. folliculorum） 41% and Demodex brevis （D. brevis） 1%]. Among patients who had ocular Demodex infestation, 69% have CD and had an average MGD score of 4; in patients without demodicosis, 15.5% had CD and had an average MGD score of 4.12. Prevalence of ocular demodicosis as determined by semi-nested PCR was 79% （D. folliculorum 78% and D. brevis 1%）.CONCLUSION： This is the first report on the prevalence of ocular demodicosis in Thailand. Patients with CD also had Demodex mites present. Semi-nested PCR is better than microscopy for Demodex infestation detection. An extensive survey with more representative samples is required to determine the prevalence in the country.

关 键 词：PREVALENCE ocular demodicosis Demodexfolliculorum Demodex brevis Thailand 

分 类 号：R779.14[医药卫生—眼科]