豆科共生关键基因表达载体的构建及其功能互补分析  

Construction and complementary analysis of the multiple legume symbiosis genes expression vectors

作　　者：李豪[1,2] 梅江鹏 张忠明[1,2] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070 [2]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《中国科技论文》2016年第24期2806-2810,共5页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20130146130003)

摘　　要：利用生物工程技术扩大根瘤菌宿主范围、提高固氮效率,实现非豆科植物共生固氮一直是生物固氮研究的热点。将豆科植物百脉根与根瘤菌共生信号通路的9个关键基因分别置于不同根组织强表达启动子下,构建了5个用于转化水稻的多基因表达载体。通过毛根转化互补豆科植物突变体,结果表明,这些基因大多数都能互补百脉根或苜蓿相应基因突变体,即多基因表达载体可用于水稻稳定转化,在水稻中搭建1条与根瘤菌共生的基因线路,为探索构建水稻共生固氮体系提供理论和技术支撑。Legumes have the capability to associate with rhizobia and fix nitrogen.Engineering nitrogen-fixing cereals has become a hot topic in biological nitrogen fixation field.Here,different promoters control expression of nine genes which are essential for Lotus japonicus to establish a symbiosis with rhizobia bacteria.Then,five multi-gene vectors are constructed,which can complement mutant leguminous plants by hairy root transformation.The results suggest that most genes are able to restore symbiotic function.These multi-gene expression vectors can be used to transfer rice（Oryza sativa）and establish a route of symbiosis signaling,which can provide a theoretical and technical foundation for setting up a rhizobium-nonlegume symbiosis in the future.

关 键 词：微生物学 生物固氮 共生基因 多基因表达载体 百脉根 水稻 

分 类 号：Q943.2[生物学—植物学]