低氧增强自噬促进舌鳞状细胞癌细胞迁移和侵袭  

Hypoxia induced autophagy and promotes the migration and invasion in tongue squamous cell carcinoma

作　　者：陈冠辉[1] 王成[1] 翁军权[1] 梁建锋[1] 李文清[1] 竺越[1] 侯劲松[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2016年第6期372-376,共5页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81572660);广东省自然科学基金(2015A030313034);广东省科技计划(2013B021800059);中山大学临床医学研究5010计划(2015018)

摘　　要：目的探讨低氧条件下对舌鳞状细胞癌(TSCC)自噬活性和迁移能力的影响。方法应用含1%O2的低氧培养箱培养TSCC UM1细胞,Western blot检测低氧诱导3、6、9、12、24 h后低氧诱导因子1α(HIF-1α)和自噬标记蛋白Beclin-1、自噬相关蛋白5(ATG5)和微管相关蛋白轻链3(LC3)的表达变化;细胞划痕实验检测低氧诱导后细胞迁移能力的改变;Transwell侵袭小室实验检测低氧诱导后细胞的侵袭能力;SPSS 13.0统计软件进行数据分析。结果低氧条件下,与对照组(0.527±0.055)相比,TSCC UM1细胞自噬体双层膜标记蛋白LC3Ⅱ的表达(1.206±0.053)增加(t=12.96,P<0.001),同时自噬相关蛋白Beclin-1表达量(1.151±0.078)较对照组(0.775±0.062)明显提高(t=6.736,P=0.001),ATG5的表达(1.231±0.06)较对照组(0.711±0.052)也明显上升(t=7.834,P=0.001)。与对照组相比,低氧诱导后细胞迁移能力(0.349±0.024)较对照组(0.788±0.037)明显增加(t=9.918,P=0.001),细胞侵袭能力(23±2)较对照组(71±4)明显增强(t=9.528,P=0.001)。结论低氧条件下可以增强TSCC细胞自噬活性,促进其迁移和侵袭。Objective To investigate the effect of hypoxia on autophagy and migration of tonguesquamous cell carcinoma（TSCC）cell line. Methods UM1 cells were cultured in hypoxia incubatorcontaining 1% oxygen concentration. Western blot was performed to examine the expression of HIF- 1α,Beclin-1,ATG5 and LC3 following 3,6,9,12 and 24 h of treatment of hypoxia. Wounding healing wasused to evaluate cell migration. Transwell assay was carried out to assess cell invasion. Statistical analysiswas performed using SPSS 13.0 software. Results After,The expression level of autophagic protein LC3Ⅱ（1.206 ± 0.053）in the hypoxia treatment group was higher than control group（0.527 ± 0.055）and bothshowed statistically significant difference（t = 12.96,P0.001）;the expression level of Beclin-1（1.151 ±0.078）in the hypoxia treatment group was higher than control group（0.775 ± 0.062）,and both showed astatistically significant difference（t=6.736,P=0.001）;the expression level of ATG5（1.231±0.06）in thehypoxia treatment group was higher than control group（0.711 ± 0.052）,was and both showed a statisticallysignificant difference（t = 7.834,P = 0.001）. The migration ability of TSCCUM1（0.349 ± 0.024）wasimproved under hypoxia in comparison to control groups（0.788±0.037）（t=9.918,P=0.001）. The invasionability of TSCCUM1（23 ± 2）was upregulated under hypoxia in comparison to control groups（71 ± 4）（t =9.528,P = 0.001）. Conclusion Hypoxia can improve autophagic activity levels and promotes the migrationand invasion in tongue squamous cell carcinoma cell line.

关 键 词：舌 癌 鳞状细胞 低氧 自噬 迁移 侵袭 

分 类 号：R739.86[医药卫生—肿瘤]