雌激素通过调节AKT信号通路活性抑制肝癌细胞的侵袭和转移  

Oestrogen inhibits invasion and metastasis of hepatocellular carcinoma MHCC97H cells by regulating the activity of AKT signaling pathway

作　　者：田长印[1] 张欣[1] 赵文学[1] 党双锁[1] 靳耀锋[2] 纪泛扑[1,3] 

机构地区：[1]西安交通大学第二附属医院感染科,陕西西安710004 [2]西安交通大学第二附属医院病理科,陕西西安710004 [3]陕西省肝脾疾病临床医学研究中心,陕西西安710004

出　　处：《南方医科大学学报》2016年第12期1621-1625,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81300322);陕西省科技公关项目(2008K09-05);西安交通大学第二附属医院人才培养专项基金(RC(GG)201501)~~

摘　　要：目的探讨雌激素对MHCC97H肝癌细胞侵袭转移的作用及其相应机制。方法利用划痕试验检测雌激素对MHCC97H肝癌细胞迁移能力的影响。Transwell小室法检测雌激素对MHCC97H肝癌细胞侵袭能力的影响。利用Western blotting法检测雌激素对MHCC97H肝癌细胞中MMP-2和MMP-9表达水平的影响。检测雌激素对MHCC97H肝癌细胞中AKT和p-AKT蛋白表达水平的影响。结果雌激素能够有效抑制MHCC97H肝癌细胞的迁移和侵袭能力,随着浓度的升高抑制能力逐渐增强,0.1μmol/L和1μmol/L浓度组MHCC97H肝癌细胞穿过基质胶到达小室底端的细胞数目分别为对照组的(68.99±15.74)%和(34.28±8.17)%(P<0.05)。雌激素能够有效抑制MHCC97H肝癌细胞中MMP-2和MMP-9的表达,当浓度为1μmol/L时,差异具有统计学意义(P<0.05)。雌激素能够有效抑制MHCC97H肝癌细胞AKT的磷酸化水平,当浓度为1μmol/L时,磷酸化水平为对照组的(90±2)%(P<0.05)。结论雌激素能够有效抑制MHCC97H肝癌细胞的迁移和侵袭,这种作用可能部分与调节AKT信号通路的活性进一步调节MMP-2和MMP-9的表达有关。然而,需要进一步的深入研究以证实这种作用关系。Objective To explore the inhibitory effect of estrogen against metastasis of human hepatocellular carcinoma MHCC97 H cells and explore the molecular mechanism. Methods The inhibitory effect of estrogen on the migration and invasion of MHCC97 H cells was evaluated with wound healing assay and Transwell assay. Western blotting was used for investigating the expression of MMP-2, MMP-9, AKT and p-AKT in the cells treated with estrogen. Results Estrogen treatment significantly inhibited the migration and invasion of MHCC97 H cells in a dose-dependent manner. Estrogen significantly down-regulated the protein expressions of MMP-2 and MMP-9 and lowered the phosphorylation level of AKT. Conclusion The anti-metastatic effect of estrogen involves inhibition of MMP-2 and MMP-9 in MHCC97 H cells probably by regulating AKT signal pathway.

关 键 词：雌激素 MHCC97H细胞 侵袭 AKT信号通路 基质金属蛋白酶 

分 类 号：R735.7[医药卫生—肿瘤]