外源性H2s对血管性痴呆模型大鼠海马组织H2s浓度和胱硫醚β-合酶表达的影响  

Effects of exogenous H2S on H2S concentration and cystathionine β-synthase expression in hippocampus in a rat model of vascular dementia

作　　者：田茂[1] 黄赟[2] 董艳军[3] 肖雁[2] 官志忠[2] 

机构地区：[1]贵州医科大学附属白云医院检验科,贵阳550058 [2]贵州医科大学分子生物学重点实验室,贵阳550004 [3]贵州省人民医院病理科,贵阳550002

出　　处：《国际脑血管病杂志》2016年第12期1091-1095,共5页International Journal of Cerebrovascular Diseases

基　　金：国家自然科学基金（81160149）;贵州省科技厅社会攻关计划（黔科合SY[2010]3076）;贵州省科技厅基金（黔科合J字[2014]2012）

摘　　要：目的探讨外源性硫化氢（hydrogen sulfide，H2S）对血管性痴呆（vascular dementia，VaD）大鼠海马组织H2s浓度和胱硫醚β-合酶（cystathionine β-synthase，CBS）表达的影响。方法采用改良四血管法制作大鼠VaD模型，应用随机数字表法分为假手术组、模型组、小剂量和大剂量NaHS组，再按照模型制作后时间进一步分为1d、7d和30d亚组。小剂量和大剂量NaHS组分别模型制作后每日腹腔注射NaHS30μmol／kg和100μmol／kg，假手术组和VaD模型组每日腹腔注射等体积生理盐水。应用Morris水迷宫实验评价大鼠学习记忆能力，应用实时免疫荧光聚合酶链反应检测海马组织CBSmRNA表达，应用蛋白质印迹法检测海马组织CBS蛋白表达。结果Morris水迷宫实验显示，模型组、小剂量和大剂量NaHS组逃避潜伏期均较假手术组显著延长（P均〈0．05），穿越平台次数均较模型组显著减少（P均〈0．05），小剂量和大剂量NaHS组逃避潜伏期均较模型组显著缩短（P均〈0．05）。模型组、小剂量和大剂量NaHS组海马H2S含量均较假手术组显著降低，但小剂量和大剂量NaHS组显著高于模型组（P均〈0．05）。模型组、小剂量和大剂量NaHS组海马CBSmRNA和蛋白表达均显著低于假手术组（P均〈0．05），且小剂量和大剂量NaHS组与模型组无显著差异。结论外源性H2S能改善VaD大鼠学习记忆能力，可能与增高海马组织H2S含量有关，但对CBS表达无影响。Objective To investigate the effect of exogenous hydrogen sulfide （H2S） on H2S concentration and cystathionine β-synthase （CBS） expression in hippocampus in a rat model of vascular dementia （VaD）. Methods A rat model of VaD was induced by using the modified four-vessel occlusion. The rats were divided into sham operation, model, low-dose and high-dose Naris groups using the random number table method. They were further redivided into one day, seven-day, and 30-day subgroups according to the time after modeling. After modeling respectively, Naris 30μmol/kg and 100 μmol/kg were injected intraperitoneally every day in the low-dose and high-dose NariS groups. The normal saline was injected intraperitoneally every day in the sham operation group and the VaD model group. Morris water maze test was used to evaluate the learning and memory ability of the rats. The expression of CBS in hippocampus was detected by real-time fluorescent polymerase chain reaction. Western Blotting was used to detect expression of CBS protein in hippocampus. Results Morris water maze test showed that the escape iatencies of the model group, low-dose and high-dose Naris groups were prolonged significantly compared with the sham operation group （P 〈0. 05）; the times of crossing the platform were decreased significantly compared with the model group （P〈0. 05）; and the escape latencies were shortened significantly in the low-dose and high-dose Naris groups compared with the model group （P 〈0. 05）. The H2S content in hippocampus was decreased significantly in the model group, low-dose and high-dose NariS groups compared with the sham operation group, but the low-dose and high-dose Naris group was significantly higher than that in the model group （all P 〈 0. 05）. The expression of CBS mRNA and protein in the model, low-dose and high-dose Naris groups was significantly lower than that of the sham operation group （all P〈 0. 05）, and there was no significant difference between the low-dose and high-dose Nar

关 键 词：痴呆 血管性 硫化氢 海马 胱硫醚Β合酶 疾病模型 动物 大鼠 

分 类 号：R749.13[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]