质谱在金属抗肿瘤化合物分子作用机理研究中的应用  

Investigations of molecular mechanism of action of metal-based anticancer complexes by mass spectrometry

作　　者：吴魁[1] 罗群[1] 赵耀[1] 郑伟[1] 汪福意[1] 

机构地区：[1]北京分子科学国家实验室,北京国家质谱中心,活体分析化学中国科学院重点实验室,中国科学院化学研究所,北京100190

出　　处：《中国科学：化学》2017年第2期233-248,共16页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21135006,21127901,21275148,21321003,21505141);国家重点基础研究发展计划(编号:2013CB531805)资助项目

摘　　要：软电离质谱,包括电喷雾质谱(ESI-MS)和基质辅助激光解吸电离质谱(MALDI-MS)已成为研究金属抗肿瘤药物与蛋白质、DNA等生物大分子相互作用最强有力的工具.质谱成像技术在组织、细胞水平上探索金属抗肿瘤化合物作用机理研究上的应用也方兴未艾.本文基于在金属抗肿瘤配合物分子作用机理研究中的工作进展,系统地总结、评述质谱在铂基和钌基金属抗肿瘤配合物分子作用机理研究中的应用,介绍质谱研究金属抗肿瘤配合物与蛋白质和DNA相互作用及作用位点的最新研究成果,并展望质谱在这一前沿交叉领域的应用前景和趋势.Soft ionization mass spectrometry,including electrospray ionization（ESI） and matrix-assistant laser desorption/ionization（MALDI） mass spectrometry,has emerged to be one of the most powerful tool for studying the interactions between metal-based anticancer complexes and biological targets such as proteins and DNA.While the application of mass spectrometry imaging for the researches in the mechanism of action of anticancer metallodrugs in vitro and in vivo is being ascendant.Based on the research progress of our group in exploring the mechanism of action of metal-based anticancer complexes,this article summarizes and reviews the applications of mass spectrometry in deciphering the antitumor mechanism of platinum- and ruthenium-based anticancer complexes,discusses the update advances in the investigation of interactions between metal-based anticancer complexes and proteins/DNA by mass spectrometry,and looks into the future application and tendency at this cutting-edge research field.

关 键 词：质谱 金属抗肿瘤药物 铂 钌 分子作用机理 

分 类 号：R979.1[医药卫生—药品] O657.63[医药卫生—药学]