miR-21在胶质瘤中的表达及其对胶质瘤增殖及迁移的影响  

Expression of mi R-21 in brain glioma and its role in proliferation and migration of U87 glioma cells

作　　者：曹英海[1] 夏春义[1] 王刚[1] 李纳新[1] 

机构地区：[1]沈阳市第一人民医院神经外科,辽宁沈阳110041

出　　处：《解剖科学进展》2017年第1期65-67,共3页Progress of Anatomical Sciences

基　　金：辽宁省科技攻关资助项目(201225020)

摘　　要：目的探讨miR-21在胶质瘤中的表达及其对胶质瘤增殖迁移的影响。方法用实时荧光定量PCR方法检测39例胶质瘤组织及其对应癌旁组织miR-21的表达;利用Lipofectamine^(TM)2000将miR-21模拟物或抑制剂及其阴性对照转染至人U87胶质瘤细胞,分别应用CCK-8法和Transwell小室法检测人胶质瘤U87细胞的增殖及迁移能力。结果胶质瘤组织中miR-21的相对表达量为4.58±1.55,癌旁组织的miR-21相对表达量为1.87±0.59,胶质瘤组织中miR-21的表达显著高于癌旁组织(P<0.01)。与对照组相比,miR-21模拟物能够显著上调胶质瘤U87细胞系的增殖以及迁移能力(P<0.01);miR-21抑制剂则能够显著降低胶质瘤U87细胞系的增殖以及迁移能力(P<0.01)。结论 miR-21可能是治疗胶质瘤的潜在靶点。Objective To study the expression of miR-21 in brain glioma and its role in proliferation and migration of U87 glioma cells. Method miR-21 expression was detected by quantitative qRT-PCR in brain glioma tissues and paraneoplastie tissues of 39 patients. LipofectamineTM2000 was used to transfect miR-21 mimics, inhibitors and its negative control into human U87 glioma cells, and then CCK-8 assay and Transwell chamber assay were applied to measure the proliferation and migration of human U87 glioma cells. Results qRT-PCR results indicated that miR-21 expression level was higher in brain glioma tissues（4.58± 1.55） than in paraneoplastic tissues（1.87 ＋ 0.59, P〈0.01）. Compared with control group, miR-21 mimics could significantly upregulate the abilities of proliferation and migration of human glioma U87 cells, and miR-21 inhibitors could significantly inhibit the abilities of proliferation, migration and migration of human glioma U87 cells. Conclusion miR-21 might be a potential target for the treatment of brain glioma.

关 键 词：U87胶质瘤细胞 MIR-21 增殖 迁移 

分 类 号：R739.41[医药卫生—肿瘤]