富氢液对脓毒血症大鼠肠黏膜损伤及TLR4/Myd88/NF-κB信号分子的影响  

Protective effect and possible mechanism of hydrogen rich saline on intestinal injury in sepsis rats

作　　者：陈克研[1] 董婉维[1] 

机构地区：[1]中国医科大学实验动物部,辽宁沈阳110122

出　　处：《解剖科学进展》2017年第1期86-88,92,共4页Progress of Anatomical Sciences

基　　金：国家自然科学青年基金(31201758);中国医科大学新教师基金(XZR20160036)

摘　　要：目的探讨富氢液(HRS)对脓毒血症大鼠肠黏膜损伤及其对TLR4/Myd88/NF-κB信号通路表达的影响。方法 SD大鼠30只,随机分为假手术组(sham组)、脓毒症组(SEP组)和富氢液处理组(C组),每组10只;Sham组仅开腹,暴露盲肠,C组和SEP组行盲肠结扎穿孔术,再分别静脉给予富氢水和等量生理盐水;三组均于模型建立后4h处死大鼠,观察小肠组织病理学结果;ELISA检测血浆中肠脂肪酸结合蛋白(I-FABP)、D-乳酸和炎症因子TNF-α、IL-6、IL-10浓度;Western blot检测肠组织TLR4、Myd88和NF-κB表达。结果富氢液可以减轻脓毒血症大鼠肠黏膜损伤,降低血浆中I-FABP、D-乳酸含量,降低炎症因子TNF-α、IL-6表达,促进IL-10升高(P<0.05),抑制肠组织中TLR4、Myd88、NF-κB表达(P<0.05)。结论富氢液对脓毒血症大鼠肠黏膜屏障有保护作用,可能与TLR4/Myd88/NF-κB信号通路有关。Objective To investigate the effect of hydrogen rich saline on sepsis rat intestinal mucosa injury and the expression of TLR4/Myd88/NF-kappa B signaling pathway. Methods 30 SD rats of clean grade, weighing 250 to 300 g, were averagely randomly divided into sham operation group（sham）, sepsis group（SEP） and hydrogen rich saline treatment group（C group）. The intestinal pathology was observed, ELISA was used to detect the content of blood intestinal fatty acid binding protein（I-FABP） and D- concentration of lactic acid, and the concentration of TNF-α and IL-6 and IL-10. Western-blot was used to determine TLR4, the expression of Myd88 and NF-κB. Results Hydrogen rich saline reduced sepsis intestinal mucosal injury, decreased the contents of I-FABP and D- lactate in plasma, decreased the expression levels of inflammatory factor TNF-αand IL-6, increased IL -10 expression（P〈0.05）, inhibited the expression of TLR4 and Myd88 and NF-kappa B in intestinal tissues（P〈0.05）. Conclusion The protective effect of hydrogen rich saline on intestinal mucosal barrier in sepsis rats might be related to the regulation of TLR4/Myd88/NF-kappa B signaling pathway.

关 键 词：富氢液 脓毒症 TLR4 炎症反应 信号通路 SD大鼠 

分 类 号：R572.3[医药卫生—消化系统]