比格犬胃底黏膜相关生物活性因子的增龄变化  

Effect of Aging on Related Biological Activity Factors in Gastric Fundic Mucosa in Beagle Dogs

作　　者：李小雯[1] 郑松柏[1] 张晓莉[1] 刘菲[1] 

机构地区：[1]复旦大学附属华东医院消化科,200040

出　　处：《胃肠病学》2017年第2期106-108,共3页Chinese Journal of Gastroenterology

基　　金：上海市科委科研计划项目(12ZR1409600)

摘　　要：背景:近年研究证实胃黏膜随增龄更易受侵袭因素的损害,既往胃黏膜老化研究多以胃窦黏膜为主,鲜有提及胃底黏膜。目的:研究比格犬胃底黏膜相关生物活性因子的增龄变化。方法:将19只健康比格犬分为中青年组(1~5岁)、低龄老年组(6~8岁)和高龄老年组(≥9岁),以TBA法测定胃底黏膜组织中MDA、LPO、MPO含量,ELISA法测定胃底黏膜组织中PTEN、TE、survivin、caspase-3、caspase-9、ZO-1、CGRP、VEGF、COX-1和COX-2含量。结果:与中青年组和低龄老年组相比,高龄老年组胃底黏膜MDA、LPO、MPO、PTEN、TE、ZO-1、CGRP、VEGF、COX-1和COX-2含量显著升高(P<0.05),caspase-3和caspase-9含量无明显差异(P>0.05)。低龄老年组和高龄老年组胃底黏膜中survivin含量显著低于中青年组(P=0.000)。结论:老年比格犬胃底黏膜相关生物活性因子含量存在不同程度的不利变化,但高龄老年组与调节黏膜下血流、黏膜再生和上皮间紧密连接等相关的生物活性因子反而升高,可能是一种"退化-代偿"现象。Background：Recent studies showed that gastric mucosa was more susceptible to injury by invasion factors with aging, however, the studies were mainly on gastric antral mucosa, fundic mucosa was rarely studied. Aims： To investigate the effect of aging on related biological activity factors in gastric fundic mucosa in beagle dogs. Methods： Nineteen beagle dogs were assigned into younger group （aged 1-5 years）, junior elderly group （aged 6-8 years） and senior elderly group （aged ≥9 years）. The contents of MDA, LPO, MPO in gastric fundic mucosa were determined by TBA method. The contents of PTEN, TE, survivin, caspase-3, caspase-9, ZO-1, CGRP, VEGF, COX-1 and COX-2 were assessed by ELISA. Results： Compared with younger group and junior elderly group, contents of MDA, LPO, MPO, PTEN, TE, ZO-1, CGRP, VEGF, COX-1 and COX-2 were significantly increased in senior elderly group （P 〈 0.05）, no significant differences in contents of caspase-3 and caspase-9 were found among the three groups （P 〉 0.05）. The content of survivin in junior elderly group and senior elderly group was significantly decreased when compared with younger group （P = 0. 000 ）. Conclusions： Disadvantaging changes of biological activity factors are found in gastric fundic mucosa in elderly beagles dogs, however, gastric mucosal blood flow, mucosa regeneration and epithelial tight junction related biological activity factors are significantly increased in senior elderly beagle dogs, which may be a phenomenon of degeneration-compensation.

关 键 词：胃底 衰老 降钙素基因相关肽 环氧合酶1 环氧合酶2 

分 类 号：R573[医药卫生—消化系统]