染色体重塑分子SNF5血清差异性表达用于早期诊断乳腺癌的初步研究  

Differential expression of chromatin-remodeling complex subunit SNF5 in serum and its role in early diagnosis of breast cancers

在线阅读下载全文

作  者:华科俊[1] 胡贤杰[1] 周美琪[2] 张星[1] 陈文忠[1] 王辉[1] 赵亲明[1] 

机构地区:[1]宁波大学医学院附属鄞州人民医院普外科,浙江宁波315040 [2]浙江大学医学院附属第二医院肿瘤外科,浙江宁波310009

出  处:《中华全科医学》2017年第3期421-423,共3页Chinese Journal of General Practice

基  金:浙江省医药卫生科技计划项目(2013KYB130)

摘  要:目的探究染色体重塑分子SNF5血清差异性表达用于早期诊断乳腺癌的可行性。方法选择2010年8月—2015年8月间于宁波大学医学院附属鄞州人民医院病理检查证实的196例乳腺癌患者(0期23例、Ⅰ期46例、Ⅱ期29例、Ⅲ期67例、Ⅳ期31例,平均年龄36.3岁)为研究对象,并选择同期在该院健康体检的64名志愿者(平均年龄37.9岁)为对照组。采用实时定量PCR检测2组外周血血清中SNF5的表达水平。采用统计学软件对2组SNF5水平进行统计学分析,并采用ROC曲线分析SNF5诊断乳腺癌的价值,进而探究染色体重塑分子SNF5血清差异性表达用于早期诊断乳腺癌的可行性。结果乳腺癌患者外周血血清中SNF5水平显著性高于对照组(P<0.05),0、Ⅰ、Ⅱ、Ⅲ、Ⅳ期乳腺癌患者SNF5水平分别是对照组的13、15、15、16、23倍(均P<0.05)。ROC曲线结果显示,SNF5诊断乳腺癌的敏感度与特异度分别为82%和93%,曲线下面积为0.8734(95%CI:0.923~0.944)。结论染色体重塑分子SNF5在乳腺癌患者中的表达明显升高,其可作为早期诊断乳腺癌肿瘤的生物标志物,具有敏感度、特异度高的优点。Objective To explore the feasibility of chromatin-remodeling complex subunit SNF5 in the early diagnosis of breast cancer. Methods A total of 196 cases of breast cancer(O stage 23 cases, Ⅰ stage 46 cases, Ⅱ stage 29 cases, Ⅲ stage 67 cases, Ⅳ stage 31 cases, average age of 36.3 ) confirmed by pathological examination in Yinzhou people' s Hospi- tal Affiliated to Ningbo University from August,2010 to August ,2015 were selected as study subject, and 64 cases of vol- unteer with health examination( average age of 37.9 ) in the same period were selected as control group. The peripheral blood serum SNF5 levels of two groups were detected with real-time quantitative PCR. The SNF5 levels of two groups were statistical analyzed by statistical software, and the diagnosis of SNF5 in breast cancer was analyzed by ROC curve to ex- plore the feasibility of SNF5 in the early diagnosis of breast cancer by chromosome remodeling. Results The peripheral blood serum level of SNF5 in breast cancer patients were significantly higher than that in the control group( P 〈 0.05 ) , and the SNF5 level of five stage breast cancer patients were 13,15,15,16 and 23 times higher than that of control group, respectively( P 〈 0.05 ). ROC curve results showed that sensitivity and specificity of SNF5 in diagnosis of in breast cancer were 82% and 93 % , the area under the curve was 0. 8734 ( 95 % confidence interval :0. 923 - 0. 944). Conclusion The high expression of SNF5 was commonly in breast cancer, and could be used as the biomarkers for early diagnosis of breast cancer with high sensitivity and specificity.

关 键 词:SNF5 乳腺癌 早期诊断 生物标志物 

分 类 号:R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象